Western Blot (WB)
(POLR2B antibody (pAb) tested by Immunoprecipitation. 10 ul of POLR2B antibody was used to immunoprecipitate POLR2B from 200 ug of HEK293 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the POLR2B antibody at a dilution of 1:500.)
Rabbit anti-Human POLR2B Polyclonal Antibody | anti-POLR2B antibody
POLR2B antibody (pAb)
Gene Names
POLR2B; RPB2; POL2RB; hRPB140
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
POLR2B; Polyclonal Antibody; POLR2B antibody (pAb); RPB2; POL2RB; hsRPB2; hRPB140; anti-POLR2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-POLR2B antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 5-10ul per IP
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
IP: 5-10ul per IP
WB: 1:500-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human POLR2B.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(POLR2B antibody (pAb) tested by Immunoprecipitation. 10 ul of POLR2B antibody was used to immunoprecipitate POLR2B from 200 ug of HEK293 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the POLR2B antibody at a dilution of 1:500.)
Western Blot (WB)
(POLR2B antibody (pAb) tested by Immunoprecipitation. 10 ul of POLR2B antibody was used to immunoprecipitate POLR2B from 200 ug of HEK293 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the POLR2B antibody at a dilution of 1:500.)
Western Blot (WB)
(POLR2B antibody (pAb) tested by Western blot. Nuclear extract of HEK293 cells (30 ug) probed with POLR2B antibody (pAb) at a dilution of 1:1,500.)
Western Blot (WB)
(POLR2B antibody (pAb) tested by Western blot. Nuclear extract of HEK293 cells (30 ug) probed with POLR2B antibody (pAb) at a dilution of 1:1,500.)
Related Product Information for anti-POLR2B antibody
Short Description: Our POLR2B antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
Background: POLR2B (polymerase (RNA) II (DNA directed) polypeptide B, 140 kDa) DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).
Product Categories/Family for anti-POLR2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,897 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB2 isoform 1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
NCBI Official Symbol
POLR2B
NCBI Official Synonym Symbols
RPB2; POL2RB; hRPB140
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB2; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II second largest subunit; RNA polymerase II subunit 2; RNA polymerase II subunit B2
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB2
Protein Family
UniProt Gene Name
POLR2B
UniProt Entry Name
RPB2_HUMAN
Similar Products
Product Notes
The POLR2B polr2b (Catalog #AAA388950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLR2B antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLR2B can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 5-10ul per IP WB: 1:500-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the POLR2B polr2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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