Mouse Phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit | Pck1 elisa kit

Mouse Phosphoenolpyruvate carboxykinase, cytosolic [GTP] ELISA Kit

Cat. Num. AAA762909

• Gene Names: PCK1; PEPCK1; PEPCKC; PEPCK-C
• Reactivity: Mouse
• Synonyms: Phosphoenolpyruvate carboxykinase; cytosolic [GTP]; Mouse Phosphoenolpyruvate carboxykinase; cytosolic [GTP] ELISA Kit; Pck1/Pepck/Phosphoenolpyruvate carboxykinase; cytosolic [GTP]/PEPCK-C/Phosphoenolpyruvate carboxylase; Pck1 elisa kit

• Reactivity: Mouse
• Sequence Length: 622

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Pck1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Pck1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Pck1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Pck1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Pck1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 187281517
• NCBI GeneID: 5105
• NCBI Accession #: NP_002582.3
• NCBI GenBank Nucleotide #: NM_002591.3
• Molecular Weight: 34,156 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase, cytosolic
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 1
• NCBI Official Symbol: PCK1
• NCBI Official Synonym Symbols: PEPCK1; PEPCKC; PEPCK-C
• NCBI Protein Information: phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Protein Family: Protein kinase
• UniProt Gene Name: PCK1
• UniProt Synonym Gene Names: PEPCK-C
• UniProt Entry Name: PCKGC_HUMAN

NCBI Description

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Protein type: Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol

Molecular Function: carboxylic acid binding; GDP binding; GTP binding; magnesium ion binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity

Biological Process: carbohydrate metabolic process; cellular response to potassium ion starvation; drug metabolic process; gluconeogenesis; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process from pyruvate; internal protein amino acid acetylation; oxaloacetate metabolic process; response to activity; response to insulin stimulus

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic