Rabbit anti-Human FTSJ3 Polyclonal Antibody | anti-FTSJ3 antibody

FTSJ3, ID (FTSJ3, Putative rRNA methyltransferase 3, Protein ftsJ homolog 3, rRNA (uridine-2'-O-)-methyltransferase 3) (Biotin)

Cat. Num. AAA6300281

• Gene Names: FTSJ3; SPB1; EPCS3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FTSJ3; Polyclonal Antibody; ID (FTSJ3; Putative rRNA methyltransferase 3; Protein ftsJ homolog 3; rRNA (uridine-2'-O-)-methyltransferase 3) (Biotin); rRNA (uridine-2'-O-)-methyltransferase 3; anti-FTSJ3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FTSJ3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FTSJ3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 623-654 amino acids from the Central region of human FTSJ3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FTSJ3 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FTSJ3 antibody detected the FTSJ3 protein (arrow).)

Related Product Information for anti-FTSJ3 antibody

Although the function of this gene is not known, the existence of this gene is supported by mRNA and EST data. A possible function of the encoded protein can be inferred from amino acid sequence similarity to the E.coli FtsJ protein and to a mouse protein possibly involved in embryogenesis.

Product Categories/Family for anti-FTSJ3 antibody

Antibodies; Enzymes; Methyltransferase

References

Olsen, J.V., et al. Cell 127(3):635-648(2006). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006). Andersen, J.S., et al. Nature 433(7021):77-83(2005). Scherl, A., et al. Mol. Biol. Cell 13(11):4100-4109(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 194097365
• NCBI GeneID: 117246
• NCBI Accession #: NP_060117.3
• NCBI GenBank Nucleotide #: NM_017647.3
• UniProt Accession #: Q8IY81; Q8N3A3; Q8WXX1; Q9BWM4; Q9NXT6; B2RCA5; D3DU22
• Molecular Weight: 96,558 Da
• NCBI Official Full Name: pre-rRNA processing protein FTSJ3
• NCBI Official Synonym Full Names: FtsJ RNA methyltransferase homolog 3
• NCBI Official Symbol: FTSJ3
• NCBI Official Synonym Symbols: SPB1; EPCS3
• NCBI Protein Information: pre-rRNA processing protein FTSJ3
• UniProt Protein Name: Putative rRNA methyltransferase 3
• Protein Family: pre-rRNA processing protein
• UniProt Gene Name: FTSJ3
• UniProt Entry Name: RRMJ3_HUMAN

NCBI Description

Although the function of this gene is not known, the existence of this gene is supported by mRNA and EST data. A possible function of the encoded protein can be inferred from amino acid sequence similarity to the E.coli FtsJ protein and to a mouse protein possibly involved in embryogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FTSJ3: Probable methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA. Belongs to the methyltransferase superfamily. RlmE family.

Protein type: Nucleolus; Methyltransferase; RNA-binding; EC 2.1.1.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: nucleolar preribosome, small subunit precursor; nucleolus; nucleus

Molecular Function: protein binding; rRNA methyltransferase activity

Biological Process: enzyme-directed rRNA 2'-O-methylation