Mouse Paxillin ELISA Kit | Pax elisa kit

Mouse Paxillin (Pax) ELISA Kit

Cat. Num. AAA2031874

• Reactivity: Mouse
• Synonyms: Paxillin; Mouse Paxillin (Pax) ELISA Kit; PXN; Pax elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Paxillin (Pax).; No significant cross-reactivity or interference between Paxillin (Pax) and analogues was observed.
• Sequence Length: 586

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paxillin (Pax) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paxillin (Pax) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for Pax elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paxillin (Pax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paxillin (Pax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paxillin (Pax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paxillin (Pax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Pax elisa kit

Signal transduction; Tumor immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 58865866
• NCBI GeneID: 360820
• NCBI Accession #: NP_001012147.1
• NCBI GenBank Nucleotide #: NM_001012147.1
• Molecular Weight: 64,019 Da
• NCBI Official Full Name: paxillin
• NCBI Official Synonym Full Names: paxillin
• NCBI Official Symbol: Pxn
• NCBI Protein Information: paxillin
• UniProt Protein Name: Paxillin
• Protein Family: Paxillin
• UniProt Gene Name: Pxn
• UniProt Entry Name: PAXI_RAT

Uniprot Description

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.

Protein type: Adaptor/scaffold; Cytoskeletal; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: cell cortex; cytoplasm; cytoskeleton; focal adhesion; lamellipodium; leading edge; nucleoplasm; plasma membrane; stress fiber

Molecular Function: beta-catenin binding; BH4 domain binding; integrin binding; protein complex binding; protein kinase binding; vinculin binding; zinc ion binding

Biological Process: activation of MAPK activity; branching morphogenesis of a tube; cell-matrix adhesion; cytoskeleton organization and biogenesis; focal adhesion formation; integrin-mediated signaling pathway; lamellipodium biogenesis; peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; regulation of cell shape; transforming growth factor beta receptor signaling pathway