Typical Testing Data/Standard Curve (for reference only)
Human Pax (Paxillin) CLIA Kit | Pax clia kit
Human Pax (Paxillin) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Pax. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Pax and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Pax, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Pax. You can calculate the concentration of Pax in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.
Protein type: Cytoskeletal; Adaptor/scaffold; Cell adhesion; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm; cytoskeleton; focal adhesion; lamellipodium; cytoplasm; leading edge; stress fiber; plasma membrane; cell cortex
Molecular Function: integrin binding; zinc ion binding; protein complex binding; beta-catenin binding; BH4 domain binding; protein kinase binding; vinculin binding
Biological Process: integrin-mediated signaling pathway; lamellipodium biogenesis; focal adhesion formation; regulation of cell shape; peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; activation of MAPK activity; positive regulation of protein kinase activity; cell-matrix adhesion; transforming growth factor beta receptor signaling pathway; cytoskeleton organization and biogenesis
Research Articles on Pax
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Product Notes
The Human Pax pxn (Catalog #AAA2532141) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2532141 CLIA Kit recognizes Human Pax. It is sometimes possible for the material contained within the vial of "Pax (Paxillin), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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