Human Paxillin ELISA Kit | Pax elisa kit

Human Paxillin ELISA Kit

Cat. Num. AAA765312

• Reactivity: Human
• Synonyms: Paxillin; Human Paxillin ELISA Kit; Pax; Pax elisa kit

• Reactivity: Human
• Sequence Length: 586

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Pax elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Pax antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Pax antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Pax amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Pax can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 58865866
• NCBI GeneID: 360820
• NCBI Accession #: NP_001012147.1
• NCBI GenBank Nucleotide #: NM_001012147.1
• Molecular Weight: 64,019 Da
• NCBI Official Full Name: paxillin
• NCBI Official Synonym Full Names: paxillin
• NCBI Official Symbol: Pxn
• NCBI Protein Information: paxillin
• UniProt Protein Name: Paxillin
• Protein Family: Paxillin
• UniProt Gene Name: Pxn
• UniProt Entry Name: PAXI_RAT

Uniprot Description

PXN: a multi-domain cytoskeletal protein involved in actin-membrane attachment that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions. Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the Crk SH2 domain. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds DDEF2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.Three alternatively-spliced isoforms have been described. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Cytoskeletal; Adaptor/scaffold

Cellular Component: cell cortex; cytoplasm; cytoskeleton; focal adhesion; lamellipodium; leading edge; nucleoplasm; plasma membrane; stress fiber

Molecular Function: beta-catenin binding; BH4 domain binding; integrin binding; protein complex binding; protein kinase binding; vinculin binding; zinc ion binding

Biological Process: activation of MAPK activity; branching morphogenesis of a tube; cell-matrix adhesion; cytoskeleton organization and biogenesis; focal adhesion formation; integrin-mediated signaling pathway; lamellipodium biogenesis; peptidyl-tyrosine phosphorylation; positive regulation of protein kinase activity; regulation of cell shape; transforming growth factor beta receptor signaling pathway