Poly A Specific Ribonuclease Recombinant Protein | PARN recombinant protein

Recombinant Poly A Specific Ribonuclease (PARN)

Cat. Num. AAA2029566

• Gene Names: PARN; DAN; DKCB6; PFBMFT4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Poly A Specific Ribonuclease; Recombinant Poly A Specific Ribonuclease (PARN); PARN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; KKF IDQVVEKIED LLQSEENKNL DLEPCTGFQR KLIYQTLSWK YPKGIHVETL ETEKKERYIVISKVD
• Sequence Length: 639

• Applicable Applications for PARN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 38.1kDa
• Accurate Molecular Mass (KD): 41kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Asn60~Leu223
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 3776076
• NCBI GeneID: 5073
• UniProt Accession #: O95453; B2RCB3; B4DDG8; B4DSB0; B4DWR4; B4E1H6
• Molecular Weight: 52,989 Da
• NCBI Official Full Name: poly(A)-specific ribonuclease
• NCBI Official Synonym Full Names: poly(A)-specific ribonuclease
• NCBI Official Symbol: PARN
• NCBI Official Synonym Symbols: DAN; DKCB6; PFBMFT4
• NCBI Protein Information: poly(A)-specific ribonuclease PARN
• UniProt Protein Name: Poly(A)-specific ribonuclease PARN
• Protein Family: Poly(A)-specific ribonuclease
• UniProt Gene Name: PARN
• UniProt Synonym Gene Names: DAN
• UniProt Entry Name: PARN_HUMAN

NCBI Description

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

PARN: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Homodimer. Interacts with KHSRP and CELF1/CUGBP1. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B. Ubiquitous. Belongs to the CAF1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; EC 3.1.13.4; Ribonuclease; RNA-binding

Chromosomal Location of Human Ortholog: 16p13

Cellular Component: cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: protein binding; poly(A)-specific ribonuclease activity; nuclease activity; mRNA 3'-UTR binding; metal ion binding; nucleotide binding; protein kinase binding

Biological Process: poly(A) tail shortening; RNA modification; mRNA catabolic process, nonsense-mediated decay; female gamete generation; gene expression; mRNA catabolic process, deadenylation-dependent decay

Disease: Pulmonary Fibrosis And/or Bone Marrow Failure, Telomere-related, 4; Dyskeratosis Congenita, Autosomal Recessive, 6