Human Presenilins-associated rhomboid-like protein ELISA Kit | PARL elisa kit

Human Presenilins-associated rhomboid-like protein, mitochondrial (PARL) ELISA Kit

Cat. Num. AAA281066

• Gene Names: PARL; PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
• Reactivity: Human
• Synonyms: Presenilins-associated rhomboid-like protein; Human Presenilins-associated rhomboid-like protein; mitochondrial (PARL) ELISA Kit; PARL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PARL. No significant cross-reactivity or interference between Human PARL and analogues was observed.

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PARL elisa kit

PARL encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms.The deduced 379-amino acid protein has a calculated molecular mass of about 42.2 kD. In vitro, PARL cDNA generated a polypeptide of about 40 kD. PARL shares significant similarity with the Drosophila development regulator Rhomboid, including 6 conserved transmembrane regions. In addition, PARL has a nonconserved N-terminal transmembrane helix.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PARL in samples. An antibody specific for PARL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 83641878
• NCBI GeneID: 55486
• NCBI Accession #: NP_001032728.1
• NCBI GenBank Nucleotide #: NM_001037639.2
• UniProt Accession #: Q9H300; Q96CQ4; Q9BTJ6; Q9P1E3
• Molecular Weight: 36,589 Da
• NCBI Official Full Name: presenilins-associated rhomboid-like protein, mitochondrial isoform 2
• NCBI Official Synonym Full Names: presenilin associated rhomboid like
• NCBI Official Symbol: PARL
• NCBI Official Synonym Symbols: PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
• NCBI Protein Information: presenilins-associated rhomboid-like protein, mitochondrial
• UniProt Protein Name: Presenilins-associated rhomboid-like protein, mitochondrial
• Protein Family: Presenilins-associated rhomboid-like protein
• UniProt Gene Name: PARL
• UniProt Synonym Gene Names: PSARL; Pbeta

NCBI Description

This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease. [provided by RefSeq, May 2016]

Uniprot Description

PSARL: a mitochondrial intramembrane protease that is associated with insulin resistance and type 2 diabetes. Induced in humans by caloric restriction. Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain. Induced in humans by caloric restriction. Interacts with PSEN1 and PSEN2. Binds OPA1. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.21.105; Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Protease

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: integral component of membrane; mitochondrial inner membrane; mitochondrion; nucleus

Molecular Function: endopeptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: membrane protein proteolysis; mitochondrial calcium ion transport; proteolysis; regulation of proteolysis