Rabbit PARL Polyclonal Antibody | anti-PARL antibody

PARL Antibody

Cat. Num. AAA150055

• Gene Names: PARL; PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: PARL Antibody is affinity chromatography purified via peptide column.
• Synonyms: PARL; Polyclonal Antibody; PARL Antibody; PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2; Presenilins-associated rhomboid-like protein; mitochondrial; Mitochondrial intramembrane cleaving protease PARL; Pbeta; presenilin associated; rhomboid-like; anti-PARL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PARL Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 379

• Applicable Applications for anti-PARL antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: PARL antibody can be used for detection of PARL by Western blot at 1 - 2 mug/mL. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PARL antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PARL.
• Buffer: PARL Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PARL antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PARL in 3T3 cells with PARL antibody at 2.50 μg/mL.)

Related Product Information for anti-PARL antibody

PARL Antibody: PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL's association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer's disease through its associations with presenilin-1 and -2. Multiple isoforms of PARL are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 143811433
• NCBI GeneID: 55486
• UniProt Accession #: Q9H300; Q96CQ4; Q9BTJ6; Q9P1E3
• Molecular Weight: 36,589 Da
• NCBI Official Full Name: Presenilins-associated rhomboid-like protein, mitochondrial
• NCBI Official Synonym Full Names: presenilin associated, rhomboid-like
• NCBI Official Symbol: PARL
• NCBI Official Synonym Symbols: PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
• NCBI Protein Information: presenilins-associated rhomboid-like protein, mitochondrial; rhomboid 7 homolog 1; mitochondrial intramembrane cleaving protease PARL; mitochondrial intramembrane-cleaving protease PARL
• UniProt Protein Name: Presenilins-associated rhomboid-like protein, mitochondrial
• Protein Family: Presenilins-associated rhomboid-like protein
• UniProt Gene Name: PARL
• UniProt Synonym Gene Names: PSARL; Pbeta
• UniProt Entry Name: PARL_HUMAN

NCBI Description

This gene encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PSARL: a mitochondrial intramembrane protease that is associated with insulin resistance and type 2 diabetes. Induced in humans by caloric restriction. Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptotic signals. Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain. Induced in humans by caloric restriction. Interacts with PSEN1 and PSEN2. Binds OPA1. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral; Protease; EC 3.4.21.105; Membrane protein, multi-pass; Mitochondrial

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: mitochondrion; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: protein binding; serine-type endopeptidase activity; endopeptidase activity

Biological Process: membrane protein proteolysis; proteolysis