Western Blot (WB)
(Figure 1: Western blot analysis using PARL mouse mAb against truncated Trx-PARL recombinant protein (1) and truncated MBP-PARL(aa112-167) recombinant protein (2).)
Mouse anti-Human PARL Monoclonal Antibody | anti-PARL antibody
PARL Antibody
Gene Names
PARL; PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
PARL; Monoclonal Antibody; PARL Antibody; Mitochondrial intramembrane cleaving protease PARL; Mitochondrial intramembrane-cleaving protease PARL; P-beta; parl; PARL_HUMAN; Pbeta; presenilin associated rhomboid-like; Presenilins associated rhomboid-like protein; PRO2207; PSARL; PSARL1; PSENIP2; RHBDS1; Rhomboid 7 homolog 1; anti-PARL antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
PARL antibody detects endogenous levels of total PARL
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
329
Applicable Applications for anti-PARL antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human PARL expressed in E. Coli
Subcellular Location
Mitochondrion Inner Membrane and Nucleus. Translocated into the nucleus by an unknown mechanism.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using PARL mouse mAb against truncated Trx-PARL recombinant protein (1) and truncated MBP-PARL(aa112-167) recombinant protein (2).)
Western Blot (WB)
(Figure 1: Western blot analysis using PARL mouse mAb against truncated Trx-PARL recombinant protein (1) and truncated MBP-PARL(aa112-167) recombinant protein (2).)
Related Product Information for anti-PARL antibody
Description: PARL: presenilin associated, rhomboid-like. This gene encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms.
Function: Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.
Subunit Structure: Interacts with PSEN1 and PSEN2. Binds OPA1.
Post-translational Modifications: P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated.
Similarity: Belongs to the peptidase S54 family.
Function: Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.
Subunit Structure: Interacts with PSEN1 and PSEN2. Binds OPA1.
Post-translational Modifications: P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated.
Similarity: Belongs to the peptidase S54 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 42 kDa
Predicted: 43 kDa
Predicted: 43 kDa
NCBI Official Full Name
presenilins-associated rhomboid-like protein, mitochondrial isoform 2
NCBI Official Synonym Full Names
presenilin associated rhomboid like
NCBI Official Symbol
PARL
NCBI Official Synonym Symbols
PSARL; PSARL1; RHBDS1; PRO2207; PSENIP2
NCBI Protein Information
presenilins-associated rhomboid-like protein, mitochondrial
UniProt Protein Name
Presenilins-associated rhomboid-like protein, mitochondrial
Protein Family
UniProt Gene Name
PARL
UniProt Synonym Gene Names
PSARL; Pbeta
NCBI Description
This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease. [provided by RefSeq, May 2016]
Uniprot Description
Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.
Research Articles on PARL
Similar Products
Product Notes
The PARL parl (Catalog #AAA9602789) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PARL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PARL parl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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