Park7 recombinant protein

Park7, 1-189 aa, Mouse, His tag, E Coli

Cat. Num. AAA206351

• Gene Names: Park7; Dj1; DJ-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Park7; 1-189 aa; Mouse; His tag; E Coli; Protein DJ-1; DJ-1; Dj1; Park7 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASKRAL VILAKGAEEM ETVIPVDVMR RAGIKVTVAG LAGKDPVQCS RDVMICPDTS LEDAKTQGPY DVVVLPGGNL GAQNLSESPM VKEILKEQES RKGLIAAICA GPTALLAHEV GFGCKVTTHP LAKDKMMNGS HYSYSESRVE KDGLILTSRG PGTSFEFALA IVEALVGKDM ANQVKAPLVL KD
• Sequence Length: 189

• Applicable Applications for Park7 recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Park7 recombinant protein

Park7 also known as protein DJ-1 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. Play an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease. Recombinant mouse Park7, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for Park7 recombinant protein

Neuroscience; Cancer

References

Taira T., et al (2004). EMBO Rep. 5 (2):213-8.; Kim R.H., et al (2005). Proc. Natl. Acad. Sci. U.S.A. 102 (14):5215-20.

NCBI and Uniprot Product Information

• NCBI GI #: 55741460
• NCBI GeneID: 57320
• NCBI Accession #: NP_065594
• NCBI GenBank Nucleotide #: NM_020569.3
• UniProt Accession #: Q99LX0; O88306; Q3THB9; Q3U509
• Molecular Weight: 22.4 kDa (212aa) confirmed by MALDI-TOF
• NCBI Official Full Name: protein/nucleic acid deglycase DJ-1
• NCBI Official Synonym Full Names: Parkinson disease (autosomal recessive, early onset) 7
• NCBI Official Symbol: Park7
• NCBI Official Synonym Symbols: Dj1; DJ-1
• NCBI Protein Information: protein/nucleic acid deglycase DJ-1
• UniProt Protein Name: Protein/nucleic acid deglycase DJ-1
• Protein Family: Protein deglycase
• UniProt Gene Name: Park7
• UniProt Synonym Gene Names: DJ-1

Uniprot Description

Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage. Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair (). Also displays an apparent glyoxalase activity that in fact reflects its deglycase activity (PubMed:22523093). Plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease; functions probably related to its primary function (PubMed:15784737, PubMed:17015834, PubMed:20800516, PubMed:21068725). It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway (PubMed:17015834, PubMed:21097510). Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death (PubMed:17766438). Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria (PubMed:20186336). Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking (PubMed:21068725). Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells (PubMed:23847046, PubMed:19276172). In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner (PubMed:22611253). Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting (PubMed:26422139). Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress (). Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity (PubMed:23792957). In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis (PubMed:26021615).