Rabbit anti-Human PTPRN Polyclonal Antibody | anti-PTPRN antibody

PTPRN, ID (PTPRN, ICA3, ICA512, Receptor-type tyrosine-protein phosphatase-like N, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA-2) (FITC)

Cat. Num. AAA6339088

• Gene Names: PTPRN; IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PTPRN; Polyclonal Antibody; ID (PTPRN; ICA3; ICA512; Receptor-type tyrosine-protein phosphatase-like N; Islet cell antigen 512; Islet cell autoantigen 3; PTP IA-2) (FITC); PTP IA-2; anti-PTPRN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PTPRN.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PTPRN antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 288-316 of human PTPRN.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS645228 in Jurkat cell line lysates (35ug/lane). This demonstrates the PTPRN antibody detected the PTPRN protein (arrow).)

Related Product Information for anti-PTPRN antibody

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus.

Product Categories/Family for anti-PTPRN antibody

Antibodies; Enzymes; Phosphatase

References

1. Yu, L., et al. J. Immunol. Methods 353 (1-2), 20-23 (2010). 2. Honeyman, M.C., et al. J. Immunol. 184(4):2204-2210(2010). 3. Weenink, S.M., et al. J. Autoimmun. 33(2):147-154(2009). 4. Burbelo, P.D., et al. Diabetes Care 31(9):1824-1826(2008). 5. Williams, A.J., et al. Diabetologia 51(8):1444-1448(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4506321
• NCBI GeneID: 5798
• NCBI Accession #: NP_002837.1
• NCBI GenBank Nucleotide #: NM_002846.3
• UniProt Accession #: Q16849; Q08319; Q53QD6; Q6NSL1; B4DK12; F5GZS3
• Molecular Weight: 96,261 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase-like N isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, N
• NCBI Official Symbol: PTPRN
• NCBI Official Synonym Symbols: IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase-like N; ICA 512; PTP IA-2; islet cell antigen 2; islet cell antigen 512; islet cell autoantigen 3; protein tyrosine phosphatase-like N
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase-like N
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRN
• UniProt Synonym Gene Names: ICA3; ICA512; R-PTP-N; ICA 512
• UniProt Entry Name: PTPRN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]

Uniprot Description

PTPRN: Implicated in neuroendocrine secretory processes. May be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking or regulated exocytosis or may have a general role in neuroendocrine functions. Seems to lack intrinsic enzyme activity. May play a role in the regulation of secretory granules via its interaction with SNTB2. Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q35-q36.1

Cellular Component: integral to membrane

Molecular Function: GTPase binding; protein binding; spectrin binding; protein tyrosine phosphatase activity

Biological Process: response to reactive oxygen species; response to cAMP; response to estrogen stimulus; insulin secretion; cytokine and chemokine mediated signaling pathway; response to glucose stimulus; protein amino acid dephosphorylation; response to insulin stimulus