Typical Testing Data/Standard Curve (for reference only)
Porcine Protease Activated Receptor 1 ELISA Kit | PAR1 elisa kit
Porcine Protease Activated Receptor 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PAR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PAR1 antibody and an PAR1-HRP conjugate. The assay sample and buffer are incubated together with PAR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAR1 concentration since PAR1 from samples and PAR1-HRP conjugate compete for the anti-PAR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAR1 from the sample, fewer sites are left to bind PAR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008]
Uniprot Description
PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.
Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; Cell development/differentiation
Chromosomal Location of Human Ortholog: 5q13
Cellular Component: Golgi apparatus; postsynaptic membrane; integral to plasma membrane; early endosome; plasma membrane; extracellular region; caveola; neuromuscular junction
Molecular Function: G-protein coupled receptor activity; protein binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; receptor binding; thrombin receptor activity
Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood coagulation; activation of MAPKK activity; negative regulation of glomerular filtration; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; response to lipopolysaccharide; negative regulation of cell proliferation; connective tissue replacement during inflammatory response; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; platelet dense granule organization and biogenesis; regulation of interleukin-1 beta production; positive regulation of cell proliferation; protein kinase C activation; response to wounding; positive regulation of smooth muscle contraction; negative regulation of neuron apoptosis; inflammatory response; establishment of synaptic specificity at neuromuscular junction; STAT protein nuclear translocation; caspase activation; platelet activation; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; tyrosine phosphorylation of STAT protein; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; regulation of blood coagulation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of calcium ion transport; blood coagulation; positive regulation of cell migration
Research Articles on PAR1
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Product Notes
The Porcine PAR1 f2r (Catalog #AAA755384) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755384 ELISA Kit recognizes Porcine PAR1. It is sometimes possible for the material contained within the vial of "Protease Activated Receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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