Bovine Protease Activated Receptor 1 ELISA Kit | PAR1 elisa kit

Bovine Protease Activated Receptor 1 ELISA Kit

Cat. Num. AAA753284

• Gene Names: F2R; TR; HTR; CF2R; PAR1; PAR-1
• Reactivity: Bovine
• Synonyms: Protease Activated Receptor 1; Bovine Protease Activated Receptor 1 ELISA Kit; PAR1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAR1 elisa kit

Principle of the assay: PAR-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAR-1 antibody and an PAR-1-HRP conjugate. The assay sample and buffer are incubated together with PAR-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAR-1 concentration since PAR-1 from samples and PAR-1-HRP conjugate compete for the anti-PAR-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAR-1 from the sample, fewer sites are left to bind PAR-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAR-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PAR1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 166362740
• NCBI GeneID: 2149
• NCBI Accession #: NP_001983.2
• NCBI GenBank Nucleotide #: NM_001992.3
• Molecular Weight: 47,441 Da
• NCBI Official Full Name: proteinase-activated receptor 1
• NCBI Official Synonym Full Names: coagulation factor II (thrombin) receptor
• NCBI Official Symbol: F2R
• NCBI Official Synonym Symbols: TR; HTR; CF2R; PAR1; PAR-1
• NCBI Protein Information: proteinase-activated receptor 1; protease-activated receptor 1
• UniProt Protein Name: Proteinase-activated receptor 1
• Protein Family: Transcription factor
• UniProt Gene Name: F2R
• UniProt Synonym Gene Names: CF2R; PAR1; TR; PAR-1
• UniProt Entry Name: PAR1_HUMAN

NCBI Description

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. [provided by RefSeq, Jul 2008]

Uniprot Description

PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; Cell development/differentiation

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: Golgi apparatus; postsynaptic membrane; integral to plasma membrane; early endosome; extracellular region; plasma membrane; caveola; neuromuscular junction

Molecular Function: G-protein coupled receptor activity; protein binding; G-protein beta-subunit binding; G-protein alpha-subunit binding; receptor binding; thrombin receptor activity

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood coagulation; negative regulation of glomerular filtration; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; positive regulation of JAK-STAT cascade; positive regulation of caspase activity; response to lipopolysaccharide; negative regulation of cell proliferation; connective tissue replacement during inflammatory response; elevation of cytosolic calcium ion concentration; platelet dense granule organization and biogenesis; positive regulation of MAPKKK cascade; regulation of interleukin-1 beta production; protein kinase C activation; positive regulation of cell proliferation; response to wounding; positive regulation of smooth muscle contraction; negative regulation of neuron apoptosis; inflammatory response; establishment of synaptic specificity at neuromuscular junction; STAT protein nuclear translocation; caspase activation; platelet activation; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of Rho protein signal transduction; positive regulation of phosphoinositide 3-kinase cascade; tyrosine phosphorylation of STAT protein; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells within a tissue; release of sequestered calcium ion into cytosol; regulation of blood coagulation; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of calcium ion transport; blood coagulation; positive regulation of cell migration