Goat Prostatic Acid Phosphatase Antibody ELISA Kit | PAP elisa kit

Goat Prostatic Acid Phosphatase Antibody ELISA Kit

Cat. Num. AAA753365

• Gene Names: ACPP; PAP; ACP3; 5'-NT; ACP-3
• Reactivity: Goat
• Synonyms: Prostatic Acid Phosphatase Antibody; Goat Prostatic Acid Phosphatase Antibody ELISA Kit; PAP elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAP elisa kit

Principle of the assay: Anti-PAP ELISA kit applies the competitive enzyme immunoassay technique utilizing PAP antigen and an Anti-PAP-HRP conjugate. The assay sample and buffer are incubated together with Anti-PAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-PAP concentration since Anti-PAP from samples and Anti-PAP-HRP conjugate compete for the PAP antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-PAP from the sample, fewer sites are left to bind Anti-PAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-PAP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PAP elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 189613
• NCBI GeneID: 55
• UniProt Accession #: P15309; Q96KY0; Q96QK9; Q96QM0; D3DNC6
• Molecular Weight: 44,566 Da
• NCBI Official Full Name: prostatic acid phosphatase
• NCBI Official Synonym Full Names: acid phosphatase, prostate
• NCBI Official Symbol: ACPP
• NCBI Official Synonym Symbols: PAP; ACP3; 5'-NT; ACP-3
• NCBI Protein Information: prostatic acid phosphatase; TMPase; 5'-nucleotidase; ecto-5'-nucleotidase; thiamine monophosphatase; prostatic acid phosphotase
• UniProt Protein Name: Prostatic acid phosphatase
• UniProt Gene Name: ACPP
• UniProt Synonym Gene Names: PAP; 5'-NT; TMPase
• UniProt Entry Name: PPAP_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Uniprot Description

ACPP: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma. Belongs to the histidine acid phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Phosphatase; EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: multivesicular body; extracellular space; Golgi cisterna; lysosomal membrane; apical part of cell; plasma membrane; integral to membrane; intracellular; vesicle membrane; nucleus; secretory granule; filopodium

Molecular Function: 5'-nucleotidase activity; identical protein binding; choline binding; acid phosphatase activity; protein homodimerization activity; phosphoric monoester hydrolase activity; thiamin phosphate phosphatase activity

Biological Process: thiamin metabolic process; dephosphorylation; positive regulation of adenosine receptor signaling pathway; nucleotide metabolic process; regulation of sensory perception of pain; adenosine metabolic process; purine base metabolic process