Typical Testing Data/Standard Curve (for reference only)
Human Serine/threonine-protein kinase PAK 1 ELISA Kit | PAK1 elisa kit
Human Serine/threonine-protein kinase PAK 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
serine/threonine protein kinase; binds and forms complex specifically with activated (GTP-bound) p21, leading to inhibiting p21 GTPase activity [RGD, Feb 2006]
Uniprot Description
PAK1: a protein kinase of the STE20 family that regulates cell motility and morphology. Critical RhoGTPase effector that regulates cytoskeleton reorganization, the JNK MAPK pathway, and nuclear signaling. Binding of Rac/cdc42 to the PBD of PAK1 causes autophosphorylation and conformational change. Phosphorylated and activated by PDK.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; PAKA subfamily
Cellular Component: axon; cytoplasm; cytosol; dendrite; Golgi apparatus; growth cone; intercellular junction; membrane; nuclear membrane; nucleus; plasma membrane; protein complex; ruffle; Z disc
Molecular Function: collagen binding; MAP kinase kinase kinase activity; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity
Biological Process: actin cytoskeleton reorganization; amygdala development; axonogenesis; branching morphogenesis of a tube; cellular response to insulin stimulus; dendrite development; MAPKKK cascade; mitotic cell cycle; neurite morphogenesis; neuromuscular junction development; positive regulation of cell migration; positive regulation of estrogen receptor signaling pathway; positive regulation of JNK activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of stress fiber formation; protein amino acid autophosphorylation; protein amino acid phosphorylation; receptor clustering; regulation of actin cytoskeleton organization and biogenesis; regulation of gene expression; regulation of MAPKKK cascade; response to hypoxia; response to organic substance; small GTPase mediated signal transduction; wound healing
Research Articles on PAK1
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Product Notes
The Human PAK1 pak1 (Catalog #AAA2887786) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2887786 ELISA Kit recognizes Human PAK1. It is sometimes possible for the material contained within the vial of "Serine/threonine-protein kinase PAK 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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