Related Product Information for PADI4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PADI4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PADI4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PADI4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PADI4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI Description
This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.6 PublicationsManual assertion based on experiment in:Ref.8
Catalytic activity: Protein L-arginine + H2O = protein L-citrulline + NH3.2 PublicationsManual assertion based on experiment in:Ref.15
Cofactor: Binds 5 calcium ions per subunit.3 PublicationsManual assertion based on experiment in:Ref.10
Enzyme regulation: Strongly Inhibited by F-amidine and N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide (Cl-amidine). These inhibitors are however not specific to PADI4 and also inhibit other members of the family (1 PublicationRef.16
Product Notes
The Human PADI4 padi4 (Catalog #
AAA706202) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA706202 ELISA Kit recognizes Human PADI4.
It is sometimes possible for the material contained within the vial of
"peptidyl arginine deiminase, type IV, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
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to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
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