Typical Testing Data/Standard Curve (for reference only)
Rat Protein-arginine deiminase type-2 ELISA Kit | Padi2 elisa kit
Rat Protein-arginine deiminase type-2 ELISA Kit
Gene Names
Padi2; Pdi2
Reactivity
Rat
Synonyms
Protein-arginine deiminase type-2; Rat Protein-arginine deiminase type-2 ELISA Kit; Peptidylarginine deiminase II; Protein-arginine deiminase type II; Padi2; Pdi; Pdi2; 3.5.3.15; Padi2 elisa kit
Reactivity
Rat
Specificity
Recombinant and natural Rat Protein-arginine deiminase type-2
Sequence Length
665
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.156-10 ng/mL
Sensitivity
<0.062 ng/mL
Intra-assay Precision
Intra-Assay CV: <=4.3%
Inter-assay Precision
Inter-Assay CV: <=7.5%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Padi2 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75,356 Da
NCBI Official Full Name
protein-arginine deiminase type-2
NCBI Official Synonym Full Names
peptidyl arginine deiminase, type II
NCBI Official Symbol
Padi2
NCBI Official Synonym Symbols
Pdi2
NCBI Protein Information
protein-arginine deiminase type-2
UniProt Protein Name
Protein-arginine deiminase type-2
Protein Family
UniProt Gene Name
Padi2
UniProt Synonym Gene Names
Pad2; Pdi; Pdi2
UniProt Entry Name
PADI2_RAT
NCBI Description
catalyzes the calcium ion dependent conversion of protein arginine residues to citrulline residues; expressed in skeletal muscle [RGD, Feb 2006]
Uniprot Description
PADI2: Catalyzes the deimination of arginine residues of proteins. Belongs to the protein arginine deiminase family.
Protein type: Hydrolase; EC 3.5.3.15
Cellular Component: cytoplasm
Molecular Function: calcium ion binding; estrogen receptor binding; protein-arginine deiminase activity
Biological Process: chromatin-mediated maintenance of transcription; estrogen receptor signaling pathway; peptidyl-citrulline biosynthetic process from peptidyl-arginine; substantia nigra development
Research Articles on Padi2
Similar Products
Product Notes
The Rat Padi2 padi2 (Catalog #AAA289974) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA289974 ELISA Kit recognizes Rat Padi2. It is sometimes possible for the material contained within the vial of "Protein-arginine deiminase type-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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