Human Polyadenylate-binding protein 3 ELISA Kit | PABPC3 elisa kit

Human Polyadenylate-binding protein 3 (PABPC3) ELISA Kit

Cat. Num. AAA282290

• Gene Names: PABPC3; PABP3; tPABP; PABPL3
• Reactivity: Human
• Synonyms: Polyadenylate-binding protein 3; Human Polyadenylate-binding protein 3 (PABPC3) ELISA Kit; PABPC3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PABPC3. No significant cross-reactivity or interference between Human PABPC3 and analogues was observed.
• Sequence Length: 631

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PABPC3 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PABPC3 in samples. An antibody specific for PABPC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPABPC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PABPC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PABPC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 45238849
• NCBI GeneID: 5042
• NCBI Accession #: NP_112241.2
• NCBI GenBank Nucleotide #: NM_030979.2
• UniProt Accession #: Q9H361; Q8NHV0; Q9H086
• Molecular Weight: 70,031 Da
• NCBI Official Full Name: polyadenylate-binding protein 3
• NCBI Official Synonym Full Names: poly(A) binding protein cytoplasmic 3
• NCBI Official Symbol: PABPC3
• NCBI Official Synonym Symbols: PABP3; tPABP; PABPL3
• NCBI Protein Information: polyadenylate-binding protein 3
• UniProt Protein Name: Polyadenylate-binding protein 3
• Protein Family: Polyadenylate-binding protein
• UniProt Gene Name: PABPC3
• UniProt Synonym Gene Names: PABP3; PABPL3; PABP-3; Poly(A)-binding protein 3

NCBI Description

Messenger RNA stability and translation initiation are extensively under the control of poly(A)-binding proteins (PABP). See PABPC1 (MIM 604679) for background information.[supplied by OMIM, Jul 2002]

Uniprot Description

PABP 3: Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1. Belongs to the polyadenylate-binding protein type-1 family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 13q12.13

Cellular Component: cytoplasm; cytosol; extracellular exosome

Molecular Function: poly(A) binding

Biological Process: mRNA metabolic process