Rabbit anti-Human RIN1 Polyclonal Antibody | anti-RIN1 antibody

RIN1 (Ras and Rab Interactor 1, Ras Interaction/Interference Protein 1, Ras Inhibitor JC99) (Biotin)

Cat. Num. AAA6392575

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RIN1; Polyclonal Antibody; RIN1 (Ras and Rab Interactor 1; Ras Interaction/Interference Protein 1; Ras Inhibitor JC99) (Biotin); anti-RIN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RIN1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 4419

• Applicable Applications for anti-RIN1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human RIN1, aa1-783 (NP_004283.2).
• Immunogen Sequence: MESPGESGAGSPGAPSPSSFTTGHLAREKPAQDPLYDVPNASGGQAGGPQRPGRVVSLRERLLLTRPVWLQLQANAAAALHMLRTEPPGTFLVRKSNTRQCQALCMRLPEASGPSFVSSHYILESPGGVSLEGSELMFPDLVQLICAYCHTRDILLLPLQLPRAIHHAATHKELEAISHLGIEFWSSSLNIKAQRGPAGGPVLPQLKARSPQELDQGTGAALCFFNPLFPGDLGPTKREKFKRSFKVRVSTETSSPLSPPAVPPPPVPVLPGAVPSQTERLPPCQLLRRESSVGYRVPAGSGPSLPPMPSLQEVDCGSPSSSEEEGVPGSRGSPATSPHLGRRRPLLRSMSAAFCSLLAPERQVGRAAAALMQDRHTAAGQLVQDLLTQVRAGPEPQELQGIRQALSRARAMLSAELGPEKLLSPKRLEHVLEKSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RIN1 rabbit polyclonal antibody. Western Blot analysis of RIN1 expression in IMR-32.)

Western Blot (WB)

(Western Blot analysis of RIN1 expression in transfected 293T cell line by RIN1 polyclonal antibody. Lane 1: RIN1 transfected lysate (84.1kD). Lane 2: Non-transfected lysate. )

Product Categories/Family for anti-RIN1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 1519314357
• NCBI GeneID: 9610
• NCBI Accession #: NM_004292.3
• NCBI GenBank Nucleotide #: NM_004292
• UniProt Accession #: Q13671
• NCBI Official Full Name: Homo sapiens Ras and Rab interactor 1 (RIN1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: Ras and Rab interactor 1
• NCBI Official Symbol: RIN1
• NCBI Protein Information: ras and Rab interactor 1
• UniProt Protein Name: Ras and Rab interactor 1
• Protein Family: RuvB-like protein
• UniProt Gene Name: RIN1
• UniProt Entry Name: RIN1_HUMAN

Uniprot Description

Rin1: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis. Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351. Expressed in all tissues examined with high levels in brain, placenta and pancreas. Belongs to the RIN (Ras interaction/interference) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rab; GEFs

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cytoskeleton; cell soma; dendrite; cytoplasm; plasma membrane

Molecular Function: protein binding; Ras GTPase binding; GTPase activator activity

Biological Process: negative regulation of synaptic plasticity; endocytosis; signal transduction; associative learning; memory; positive regulation of GTPase activity