Protein Disulfide Isomerase (PDI) Recombinant Protein | PDI recombinant protein

Recombinant Protein Disulfide Isomerase (PDI)

Cat. Num. AAA2009292

• Gene Names: P4hb; PDI; PDIR
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Disulfide Isomerase (PDI); Recombinant Protein Disulfide Isomerase (PDI); PDI recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-DY DGKLSNFKKA AEGFKGKILF IFIDSDHTDN QRILEFFGLK KEECPAVRLI TLEEEMTKYK PESDELTAEK ITQFCHHFLE GKIKPHLMSQ ELPEDWDKQP VKVLVGKNFE EVAFDEKKNV FVEFYAPWCG HCKQLAPIWD KLGETYKDHE NIVIAKMDST ANEVEAVKVH SFPTLKFFPA SADRTVIDYN GERTLDGFKKFLESGGQDGA GDNDDLDLEE ALEPDMEEDD DQKAVKD
• Sequence Length: 508

• Applicable Applications for PDI recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asp269~Asp507 (Accession # P04785) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Cell membrane; Peripheral membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6981324
• NCBI GeneID: 25506
• NCBI Accession #: NP_037130.1
• NCBI GenBank Nucleotide #: NM_012998.1
• UniProt Accession #: P04785; P13700
• Molecular Weight: 59.1kDa
• NCBI Official Full Name: protein disulfide-isomerase
• NCBI Official Synonym Full Names: prolyl 4-hydroxylase, beta polypeptide
• NCBI Official Symbol: P4hb
• NCBI Official Synonym Symbols: PDI; PDIR
• NCBI Protein Information: protein disulfide-isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide)
• UniProt Protein Name: Protein disulfide-isomerase
• UniProt Gene Name: P4hb
• UniProt Synonym Gene Names: Pdia1; PDI
• UniProt Entry Name: PDIA1_RAT

NCBI Description

may be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]

Uniprot Description

PDIA1: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1. Binds to CD4, and upon HIV-1 binding to the cell membrane, is part of a P4HB/PDI-CD4-CXCR4-gp120 complex. Belongs to the protein disulfide isomerase family.

Protein type: EC 5.3.4.1; Nuclear receptor co-regulator; Isomerase; Endoplasmic reticulum; Oxidoreductase

Cellular Component: focal adhesion; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; ER-Golgi intermediate compartment; plasma membrane

Molecular Function: enzyme binding; procollagen-proline 4-dioxygenase activity; protein heterodimerization activity; protein disulfide isomerase activity; peptidyl-proline 4-dioxygenase activity

Biological Process: protein folding; cell redox homeostasis; peptidyl-proline hydroxylation to 4-hydroxy-L-proline