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Golgi Protein 73 (GP73) Recombinant Protein | GP73 recombinant protein
Recombinant Golgi Protein 73 (GP73)
Gene Names
Golm1; GP73; Golph2; AW125446; PSEC0257; 2310001L02Rik; D030064E01Rik
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Golgi Protein 73 (GP73); Recombinant Golgi Protein 73 (GP73); GP73 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SLQQDIFQ FQKNQTSLEK KFSYDLNQCI SQMTEVKEQC DERIEEVIRK RNEAPGSRDL AETNNQHQQA LKPQPKLQEE VPSEEQMPQE KGDVPRNKSQ IPAPNSESLG LKPQVQNEET NEIQAVGEEH QQASIQGQAV ADGTRVGAEK LDQHTQLPAG LLARPEEDSQ YPEREQLVIR DRQEQQRASE EGGGQKNPGD EYDMDENEAE SEREKQAALA GNDRNINVLN ADAQKRGIIN VPVGSERQS
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SLQQDIFQ FQKNQTSLEK KFSYDLNQCI SQMTEVKEQC DERIEEVIRK RNEAPGSRDL AETNNQHQQA LKPQPKLQEE VPSEEQMPQE KGDVPRNKSQ IPAPNSESLG LKPQVQNEET NEIQAVGEEH QQASIQGQAV ADGTRVGAEK LDQHTQLPAG LLARPEEDSQ YPEREQLVIR DRQEQQRASE EGGGQKNPGD EYDMDENEAE SEREKQAALA GNDRNINVLN ADAQKRGIIN VPVGSERQS
Sequence Length
393
Applicable Applications for GP73 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ser133~Ser379 (Accession # Q91XA2) with two N-terminal Tags, His-tag and GST-tag
Subcellular Location
Golgi apparatus, cis-Golgi network membrane; Single-pass type II membrane protein.
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
5.2
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59.8kDa
NCBI Official Full Name
Golgi membrane protein 1
NCBI Official Synonym Full Names
golgi membrane protein 1
NCBI Official Symbol
Golm1
NCBI Official Synonym Symbols
GP73; Golph2; AW125446; PSEC0257; 2310001L02Rik; D030064E01Rik
NCBI Protein Information
Golgi membrane protein 1; golgi phosphoprotein 2; golgi membrane protein GP73
UniProt Protein Name
Golgi membrane protein 1
UniProt Gene Name
Golm1
UniProt Synonym Gene Names
Golph2
UniProt Entry Name
GOLM1_MOUSE
Uniprot Description
GOLM1: Unknown. Cellular response protein to viral infection. Belongs to the GOLM1/CASC4 family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Membrane protein, integral
Cellular Component: Golgi apparatus; extracellular space; membrane; integral to membrane
Biological Process: regulation of lipid metabolic process; nuclear organization and biogenesis
Research Articles on GP73
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Product Notes
The GP73 golm1 (Catalog #AAA2010132) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Golgi Protein 73 (GP73) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the GP73 golm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -SLQQDIFQ FQKNQTSLEK KFSYDLNQCI SQMTEVKEQC DERIEEVIRK RNEAPGSRDL AETNNQHQQA LKPQPKLQEE VPSEEQMPQE KGDVPRNKSQ IPAPNSESLG LKPQVQNEET NEIQAVGEEH QQASIQGQAV ADGTRVGAEK LDQHTQLPAG LLARPEEDSQ YPEREQLVIR DRQEQQRASE EGGGQKNPGD EYDMDENEAE SEREKQAALA GNDRNINVLN ADAQKRGIIN VPVGSERQS. It is sometimes possible for the material contained within the vial of "Golgi Protein 73 (GP73), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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