Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OT-R. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forOT-R and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain OT-R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of OT-R in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
G-protein coupled receptor for the peptide hormone, oxytocin [RGD, Feb 2006]
Uniprot Description
OXTR: Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1
Cellular Component: apical plasma membrane; cell-cell adherens junction; cytosol; integral to plasma membrane; intracellular; microvillus
Molecular Function: oxytocin receptor activity; peptide hormone binding; protein binding; vasopressin receptor activity
Biological Process: cellular response to hormone stimulus; eating behavior; elevation of cytosolic calcium ion concentration; female pregnancy; G-protein coupled receptor protein signaling pathway; gut development; heart development; lactation; maternal behavior; maternal process involved in parturition; memory; positive regulation of blood pressure; positive regulation of synaptic transmission, GABAergic; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; positive regulation of vasoconstriction; regulation of systemic arterial blood pressure by vasopressin; response to amphetamine; response to anoxia; response to cocaine; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to organic cyclic substance; response to peptide hormone stimulus; response to progesterone stimulus; response to steroid hormone stimulus; sleep; social behavior; sperm ejaculation; suckling behavior; telencephalon development
Research Articles on Oxtr
Similar Products
Product Notes
The Rat Oxtr oxtr (Catalog #AAA288722) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288722 ELISA Kit recognizes Rat Oxtr. It is sometimes possible for the material contained within the vial of "Oxytocin receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.