Mouse Dynein heavy chain 17, axonemal ELISA Kit | Dnah17 elisa kit

Mouse Dynein heavy chain 17, axonemal ELISA Kit

Cat. Num. AAA288234

• Gene Names: Dnah17; Gm1178; Dnahc17; Dnahcl1; mKIAA3028; 2810003K23Rik
• Reactivity: Mouse
• Synonyms: Dynein heavy chain 17; axonemal; Mouse Dynein heavy chain 17; axonemal ELISA Kit; Dnah17 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Dynein heavy chain 17, axonemal. No significant cross-reactivity or interference was observed.
• Sequence Length: 4462

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.084 ng/mL
• Intra-Assay CV: <=5.2%
• Inter-Assay CV: <=7.3%
• Recovery: 93%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Dnah17 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein heavy chain 17, axonemal. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dynein heavy chain 17, axonemal and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dynein heavy chain 17, axonemal, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Dynein heavy chain 17, axonemal in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 172045717
• NCBI GeneID: 69926
• UniProt Accession #: Q69Z23; O08820
• Molecular Weight: 440,772 Da
• NCBI Official Full Name: Dynein heavy chain 17, axonemal
• NCBI Official Synonym Full Names: dynein, axonemal, heavy chain 17
• NCBI Official Symbol: Dnah17
• NCBI Official Synonym Symbols: Gm1178; Dnahc17; Dnahcl1; mKIAA3028; 2810003K23Rik
• NCBI Protein Information: dynein heavy chain 17, axonemal
• UniProt Protein Name: Dynein heavy chain 17, axonemal
• Protein Family: Dynein
• UniProt Gene Name: Dnah17
• UniProt Synonym Gene Names: Dnahc17; Kiaa3028
• UniProt Entry Name: DYH17_MOUSE

Uniprot Description

DNAH17: Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly. Belongs to the dynein heavy chain family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding; Motility/polarity/chemotaxis

Cellular Component: cell projection; cilium; cytoplasm; cytoskeleton; dynein complex; microtubule

Molecular Function: ATP binding; ATPase activity; microtubule motor activity; motor activity; nucleotide binding; protein binding

Biological Process: microtubule-based movement