Typical Testing Data/Standard Curve
Bovine Osterix ELISA Kit | OSX elisa kit
Bovine Osterix ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay: Osterix ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-Osterix antibody and an Osterix-HRP conjugate. The assay sample and buffer are incubated together with Osterix-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Osterix concentration since Osterix from samples and Osterix-HRP conjugate compete for the anti-Osterix antibody binding site. Since the number of sites is limited, as more sites are occupied by Osterix from the sample, fewer sites are left to bind Osterix-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Osterix concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
osterix: Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences. Defects in SP7 are the cause of osteogenesis imperfecta type 11 (OI11). A connective tissue disorder characterized by bone fragility, low bone mass, recurrent fractures, mild bone deformations, generalized osteoporosis, delayed teeth eruption, no dentinogenesis imperfecta, normal hearing, and white sclerae. Belongs to the Sp1 C2H2-type zinc-finger protein family.
Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: cytoplasm; nucleus
Molecular Function: DNA binding; DEAD/H-box RNA helicase binding; metal ion binding
Biological Process: osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
Disease: Osteogenesis Imperfecta, Type Xii
Similar Products
Product Notes
The Bovine OSX sp7 (Catalog #AAA748647) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA748647 ELISA Kit recognizes Bovine OSX. It is sometimes possible for the material contained within the vial of "Osterix, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
