Rabbit anti-Human, Mouse USP13 Polyclonal Antibody | anti-USP13 antibody

USP13, ID (Ubiquitin Carboxyl-terminal Hydrolase 13, Ubiquitin Thiolesterase 13, Ubiquitin-specific Processing Protease 13, Deubiquitinating Enzyme 13, Isopeptidase T-3, ISOT-3, ISOT3) (AP)

Cat. Num. AAA6505134

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: USP13; Polyclonal Antibody; ID (Ubiquitin Carboxyl-terminal Hydrolase 13; Ubiquitin Thiolesterase 13; Ubiquitin-specific Processing Protease 13; Deubiquitinating Enzyme 13; Isopeptidase T-3; ISOT-3; ISOT3) (AP); anti-USP13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human USP13. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-USP13 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human USP13 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-USP13 Pab is used in Western blot to detect USP13 in HeLa cell lysate.)

Testing Data

(Detection of USP13 in HeLa cells expressing exogenous USP13 by anti-USP13 Pab. (Data provided by Amy Chen, Burnham Institute for Medical Research))

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-USP13 antibody

Antibodies; Ubiquitin

References

1. Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003). 2. D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998). 3. Timms, K.M., et al., Gene 217(1-2), 101-106 (1998).