Norwalk virus Capsid protein VP1 (ORF2) Recombinant Protein | ORF2 recombinant protein

Recombinant Norwalk virus Capsid protein VP1 (ORF2)

Cat. Num. AAA1291347

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Norwalk virus Capsid protein VP1 (ORF2); Recombinant Norwalk virus Capsid protein VP1 (ORF2); p59; ORF2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-530aa; Full Length
• Sequence: MMMASKDATSSVDGASGAGQLVPEVNASDPLAMDPVAGSSTAVATAGQVNPIDPWIINNFVQAPQGEFTISPNNTPGDVLFDLSLGPHLNPFLLHLSQMYNGWVGNMRVRIMLAGNAFTAGKIIVSCIPPGFGSHNLTIAQATLFPHVIADVRTLDPIEVPLEDVRNVLFHNNDRNQQTMRLVCMLYTPLRTGGGTGDSFVVAGRVMTCPSPDFNFLFLVPPTVEQKTRPFTLPNLPLSSLSNSRAPLPISSMGISPDNVQSVQFQNGRCTLDGRLVGTTPVSLSHVAKIRGTSNGTVINLTELDGTPFHPFEGPAPIGFPDLGGCDWHINMTQFGHSSQTQYDVDTTPDTFVPHLGSIQANGIGSGNYVGVLSWISPPSHPSGSQVDLWKIPNYGSSITEATHLAPSVYPPGFGEVLVFFMSKMPGPGAYNLPCLLPQEYISHLASEQAPTVGEAALLHYVDPDTGRNLGEFKAYPDGFLTCVPNGASSGPQQLPINGVFVFVSWVSRFYQLKPVGTASSARGRLGLRR
• Sequence Length: 530

• Production Note: Special Offer: The Mammalian-Cell host-expressed protein is manufactured from a stock plasmid containing the protein gene. Mammalian-Cellhost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The Mammalian-Cell host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select Mammalian-Cell host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for ORF2 recombinant protein

Capsid protein self assbles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assbled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding histo-blood group antigens present on gastroduodenal epithelial cells.

References

Sequence and genomic organization of Norwalk virus.Jiang X., Wang M., Wang K., Estes M.K.Virology 195:51-61(1993) Specific proteolytic cleavage of recombinant Norwalk virus capsid protein.Hardy M.E., White L.J., Ball J.M., Estes M.K.J. Virol. 69:1693-1698(1995) Attachment and entry of recombinant Norwalk virus capsids to cultured human and animal cell lines.White L.J., Ball J.M., Hardy M.E., Tanaka T.N., Kitamoto N., Estes M.K.J. Virol. 70:6589-6597(1996) Biochemical characterization of a smaller form of recombinant Norwalk virus capsids assembled in insect cells.White L.J., Hardy M.E., Estes M.K.J. Virol. 71:8066-8072(1997) Norwalk virus binds to histo-blood group antigens present on gastroduodenal epithelial cells of secretor individuals.Marionneau S., Ruvoen N., Le Moullac-Vaidye B., Clement M., Cailleau-Thomas A., Ruiz-Palacois G., Huang P., Jiang X., Le Pendu J.Gastroenterology 122:1967-1977(2002) The 3' end of Norwalk virus mRNA contains determinants that regulate the expression and stability of the viral capsid protein VP1 a novel function for the VP2 protein.Bertolotti-Ciarlet A., Crawford S.E., Hutson A.M., Estes M.K.J. Virol. 77:11603-11615(2003) Noroviruses bind to human ABO, Lewis, and secretor histo-blood group antigens identification of 4 distinct strain-specific patterns.Huang P., Farkas T., Marionneau S., Zhong W., Ruvoen-Clouet N., Morrow A.L., Altaye M., Pickering L.K., Newburg D.S., LePendu J., Jiang X.J. Infect. Dis. 188:19-31(2003) C-terminal arginine cluster is essential for receptor binding of norovirus capsid protein.Tan M., Meller J., Jiang X.J. Virol. 80:7322-7331(2006) Norovirus protein structure and function.Hardy M.E.FEMS Microbiol. Lett. 253:1-8(2005) X-ray crystallographic structure of the Norwalk virus capsid.Prasad B.V.V., Hardy M.E., Dokland T., Bella J., Rossmann M.G., Estes M.K.Science 286:287-290(1999)

NCBI and Uniprot Product Information

• NCBI GI #: 106060736
• NCBI GeneID: 1491972
• NCBI Accession #: NP_056821.2
• NCBI GenBank Nucleotide #: NC_001959.2
• UniProt Accession #: Q83884
• Molecular Weight: 58.6 kDa
• NCBI Official Full Name: 58 kd capsid protein
• NCBI Official Symbol: NVgp2
• NCBI Protein Information: 58 kd capsid protein
• UniProt Protein Name: Capsid protein VP1
• Protein Family: Protein
• UniProt Gene Name: ORF2
• UniProt Synonym Gene Names: CP; p30
• UniProt Entry Name: CAPSD_NVN68

Uniprot Description

Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding histo-blood group antigens present on gastroduodenal epithelial cells.