Mouse N-glycosylase/DNA lyase ELISA Kit | Ogg1 elisa kit

Mouse N-glycosylase/DNA lyase ELISA Kit

Cat. Num. AAA289739

• Gene Names: Ogg1; Mmh
• Reactivity: Mouse
• Synonyms: N-glycosylase/DNA lyase; Mouse N-glycosylase/DNA lyase ELISA Kit; Ogg1; Ogg1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse N-glycosylase/DNA lyase. No significant cross-reactivity or interference was observed.
• Sequence Length: 345

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 39.02 pg/mL
• Intra-Assay CV: <=6.8%
• Inter-Assay CV: <=8.7%
• Recovery: 85%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ogg1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse N-glycosylase/DNA lyase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-glycosylase/DNA lyase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for N-glycosylase/DNA lyase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain N-glycosylase/DNA lyase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of N-glycosylase/DNA lyase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 187960092
• NCBI GeneID: 18294
• NCBI Accession #: NP_035087.3
• NCBI GenBank Nucleotide #: NM_010957.4
• UniProt Accession #: O08760; O08733; O08910; O08991; O35617; O35915; Q9QXE8
• Molecular Weight: 38,883 Da
• NCBI Official Full Name: N-glycosylase/DNA lyase
• NCBI Official Synonym Full Names: 8-oxoguanine DNA-glycosylase 1
• NCBI Official Symbol: Ogg1
• NCBI Official Synonym Symbols: Mmh
• NCBI Protein Information: N-glycosylase/DNA lyase
• UniProt Protein Name: N-glycosylase/DNA lyase
• Protein Family: N-glycosylase/DNA lyase
• UniProt Gene Name: Ogg1
• UniProt Synonym Gene Names: AP lyase
• UniProt Entry Name: OGG1_MOUSE

Uniprot Description

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; Lyase; DNA repair, damage; EC 4.2.99.18

Cellular Component: mitochondrion; nuclear matrix; nuclear speck; nucleoplasm; nucleus; protein complex

Molecular Function: 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity; catalytic activity; damaged DNA binding; DNA binding; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; DNA-3-methyladenine glycosylase III activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; lyase activity; microtubule binding; oxidized base lesion DNA N-glycosylase activity; oxidized purine base lesion DNA N-glycosylase activity; oxidized purine DNA binding; purine-specific mismatch base pair DNA N-glycosylase activity; single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity

Biological Process: aging; base-excision repair; DNA repair; metabolic process; negative regulation of apoptosis; nucleotide-excision repair; nucleotide-excision repair, DNA incision; regulation of protein import into nucleus, translocation; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to estradiol stimulus; response to oxidative stress; response to radiation