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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant LIPG protein (Right) or empty vector (Left) detected with LIPG antibody)

Mouse LIPG Monoclonal Antibody | anti-LIPG antibody

LIPG antibody

Gene Names
LIPG; EL; EDL; PRO719
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
LIPG antibody was purified by affinity chromatography.
Synonyms
LIPG; Monoclonal Antibody; LIPG antibody; Monoclonal LIPG; Anti-LIPG; Endothelial lipase; EDL; Lipoprotein lipase H; PRO719; anti-LIPG antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6B9
Purity/Purification
LIPG antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
500
Applicable Applications for anti-LIPG antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
FC: 1:100
WB: 1:2000
Biological Significance
LIPG has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family.
Cross-Reactivity
Human
Immunogen
LIPG antibody was raised in mouse using a full length recombinant protein of human LIPG (NP_006024) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant LIPG protein (Right) or empty vector (Left) detected with LIPG antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant LIPG protein (Right) or empty vector (Left) detected with LIPG antibody)
Related Product Information for anti-LIPG antibody
Mouse monoclonal LIPG antibody
Product Categories/Family for anti-LIPG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,362 Da
NCBI Official Full Name
endothelial lipase isoform 1
NCBI Official Synonym Full Names
lipase, endothelial
NCBI Official Symbol
LIPG
NCBI Official Synonym Symbols
EL; EDL; PRO719
NCBI Protein Information
endothelial lipase
UniProt Protein Name
Endothelial lipase
Protein Family
UniProt Gene Name
LIPG
UniProt Synonym Gene Names
EDL; EL
UniProt Entry Name
LIPE_HUMAN

NCBI Description

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phospholipase; Secreted; EC 3.1.1.3; Secreted, signal peptide; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome

Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis

Research Articles on LIPG

Similar Products

Product Notes

The LIPG lipg (Catalog #AAA834282) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LIPG can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). FC: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the LIPG lipg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIPG, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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