Typical Testing Data/Standard Curve (for reference only)
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Mouse Occludin ELISA Kit | OCLN elisa kit
Mouse Occludin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: OCLN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-OCLN antibody and an OCLN-HRP conjugate. The assay sample and buffer are incubated together with OCLN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OCLN concentration since OCLN from samples and OCLN-HRP conjugate compete for the anti-OCLN antibody binding site. Since the number of sites is limited, as more sites are occupied by OCLN from the sample, fewer sites are left to bind OCLN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OCLN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
Uniprot Description
occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 5q13.1
Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; plasma membrane; integral to membrane; intercellular junction; cytoplasmic vesicle; cytosol; cell junction
Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity
Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; protein complex assembly; cell structure disassembly during apoptosis
Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
Research Articles on OCLN
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Product Notes
The Mouse OCLN ocln (Catalog #AAA729514) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729514 ELISA Kit recognizes Mouse OCLN. It is sometimes possible for the material contained within the vial of "Occludin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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