Rat Nucleoporin 214kDa ELISA Kit | NUP214 elisa kit

Rat Nucleoporin 214kDa ELISA Kit

Cat. Num. AAA730533

• Gene Names: NUP214; CAN; CAIN; N214; p250; D9S46E
• Reactivity: Rat
• Synonyms: Nucleoporin 214kDa; Rat Nucleoporin 214kDa ELISA Kit; NUP214 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This NUP214 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NUP214. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NUP214 elisa kit

Principle of the assay: NUP214 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NUP214 antibody and an NUP214-HRP conjugate. The assay sample and buffer are incubated together with NUP214-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP214 concentration since NUP214 from samples and NUP214-HRP conjugate compete for the anti-NUP214 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP214 from the sample, fewer sites are left to bind NUP214-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUP214 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NUP214 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 33946327
• NCBI GeneID: 8021
• NCBI Accession #: NP_005076.3
• NCBI GenBank Nucleotide #: NM_005085.3
• Molecular Weight: 215,401 Da
• NCBI Official Full Name: nuclear pore complex protein Nup214
• NCBI Official Synonym Full Names: nucleoporin 214kDa
• NCBI Official Symbol: NUP214
• NCBI Official Synonym Symbols: CAN; CAIN; N214; p250; D9S46E
• NCBI Protein Information: nuclear pore complex protein Nup214; CAN protein, putative oncogene
• UniProt Protein Name: Nuclear pore complex protein Nup214
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP214
• UniProt Synonym Gene Names: CAIN; CAN; KIAA0023
• UniProt Entry Name: NU214_HUMAN

NCBI Description

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

NUP214: May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex. A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene. A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear export; Nucleoporin; Oncoprotein

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; focal adhesion; intracellular membrane-bound organelle; nuclear pore; nucleus; cytosol

Molecular Function: protein binding; transporter activity; nucleocytoplasmic transporter activity

Biological Process: viral reproduction; regulation of cell cycle; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; viral infectious cycle; glucose transport; mRNA export from nucleus; protein import into nucleus; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; protein export from nucleus; mitotic cell cycle; transmembrane transport

Disease: Leukemia, Acute Lymphoblastic