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SDS-Page

Nucleoporin 205kDa (NUP205) Recombinant Protein | NUP205 recombinant protein

Recombinant Nucleoporin 205kDa (NUP205)

Gene Names
NUP205; C7orf14
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Nucleoporin 205kDa (NUP205); Recombinant Nucleoporin 205kDa (NUP205); NUP205 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DDNVEG DKVSKKDEIE LAMQQICANV MEYCQSLMLQ SSPTFQHAVC LFTPSLSETV NRDGPRQDTQ APVVPYWRLP GLGIIIYLLK QSANDFFSYY DSHRQSVSKL QNVEQLPPDE IKELCQSVMP AGVDKISTAQ KYVLARRRLV KVINNRAKLL SLCSFIIETC LFILWRHLEY YLLHCMPTDS QDSLFASRTL FKSRRLQDSF ASETNLDFRS GLAIVSQHDL DQLQADAINA FGESLQKKLL DIEGLYSKVRSRYSFIQALV
Sequence Length
2012
Applicable Applications for NUP205 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Asp1735~Val2000 (Accession # Q92621) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Nucleus membrane; Peripheral membrane protein. Nucleus, nuclear pore complex
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.9
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34.2kDa
NCBI Official Full Name
nuclear pore complex protein Nup205
NCBI Official Synonym Full Names
nucleoporin 205kDa
NCBI Official Symbol
NUP205
NCBI Official Synonym Symbols
C7orf14
NCBI Protein Information
nuclear pore complex protein Nup205; nucleoporin Nup205; 205 kDa nucleoporin
UniProt Protein Name
Nuclear pore complex protein Nup205
UniProt Gene Name
NUP205
UniProt Synonym Gene Names
C7orf14; KIAA0225
UniProt Entry Name
NU205_HUMAN

Uniprot Description

NUP205: Interacts with NUP93 and NUP35/NUP53.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: nucleoplasm; nuclear membrane; membrane; cytoplasm; nuclear envelope; nuclear pore

Molecular Function: protein binding; structural constituent of nuclear pore

Biological Process: nuclear pore complex assembly; mRNA transport; viral reproduction; protein import into nucleus, docking; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; nucleocytoplasmic transport; viral infectious cycle; glucose transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport

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Product Notes

The NUP205 nup205 (Catalog #AAA2018565) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nucleoporin 205kDa (NUP205) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the NUP205 nup205 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DDNVEG DKVSKKDEIE LAMQQICANV MEYCQSLMLQ SSPTFQHAVC LFTPSLSETV NRDGPRQDTQ APVVPYWRLP GLGIIIYLLK QSANDFFSYY DSHRQSVSKL QNVEQLPPDE IKELCQSVMP AGVDKISTAQ KYVLARRRLV KVINNRAKLL SLCSFIIETC LFILWRHLEY YLLHCMPTDS QDSLFASRTL FKSRRLQDSF ASETNLDFRS GLAIVSQHDL DQLQADAINA FGESLQKKLL DIEGLYSKVR SRYSFIQALV. It is sometimes possible for the material contained within the vial of "Nucleoporin 205kDa (NUP205), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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