Rat Nucleoporin 205kDa ELISA Kit | NUP205 elisa kit

Rat Nucleoporin 205kDa ELISA Kit

Cat. Num. AAA720684

• Gene Names: NUP205; C7orf14
• Reactivity: Rat
• Synonyms: Nucleoporin 205kDa; Rat Nucleoporin 205kDa ELISA Kit; NUP205 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This NUP205 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NUP205. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NUP205 elisa kit

Principle of the assay: NUP205 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NUP205 antibody and an NUP205-HRP conjugate. The assay sample and buffer are incubated together with NUP205-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP205 concentration since NUP205 from samples and NUP205-HRP conjugate compete for the anti-NUP205 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP205 from the sample, fewer sites are left to bind NUP205-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUP205 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NUP205 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 57634534
• NCBI GeneID: 23165
• NCBI Accession #: NP_055950.1
• NCBI GenBank Nucleotide #: NM_015135.2
• Molecular Weight: 227,922 Da
• NCBI Official Full Name: nuclear pore complex protein Nup205
• NCBI Official Synonym Full Names: nucleoporin 205kDa
• NCBI Official Symbol: NUP205
• NCBI Official Synonym Symbols: C7orf14
• NCBI Protein Information: nuclear pore complex protein Nup205; nucleoporin Nup205; 205 kDa nucleoporin
• UniProt Protein Name: Nuclear pore complex protein Nup205
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP205
• UniProt Synonym Gene Names: C7orf14; KIAA0225
• UniProt Entry Name: NU205_HUMAN

Uniprot Description

NUP205: Interacts with NUP93 and NUP35/NUP53.

Protein type: Nucleoporin

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: nucleoplasm; nuclear membrane; membrane; cytoplasm; nuclear pore; nuclear envelope

Molecular Function: protein binding; structural constituent of nuclear pore

Biological Process: nuclear pore complex assembly; mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; protein import into nucleus, docking; mitotic nuclear envelope disassembly; pathogenesis; nucleocytoplasmic transport; viral infectious cycle; glucose transport; hexose transport; carbohydrate metabolic process; gene expression; viral transcription; mitotic cell cycle; transmembrane transport