Nudix Type Motif 1 Recombinant Protein | NUDT1 recombinant protein

Recombinant Human Nudix Type Motif 1

Cat. Num. AAA144300

• Gene Names: NUDT1; MTH1
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Nudix Type Motif 1; Recombinant Human Nudix Type Motif 1; NUDT1 Human; Nudix Type Motif 1 Human Recombinant; 7; 8-dihydro-8-oxoguanine triphosphatase; 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1; NUDT1; MTH1; NUDT1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The NUDT1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV
• Sequence Length: 156

• Preparation and Storage: NUDT1 should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for NUDT1 recombinant protein

Description: NUDT1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 176 amino acids (1-156 a.a.) and having a molecular mass of 20.1kDa. The NUDT1 is purified by proprietary chromatographic techniques.

Introduction: NUDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. NUDT1 is found mostly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Moreover, NUDT1 is expressed at much higher levels in proliferating cells than in resting cells. Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations which may result in carcinogenesis or neurodegeneration.

Product Categories/Family for NUDT1 recombinant protein

Enzymes; Nudix Type Motif

NCBI and Uniprot Product Information

• NCBI GI #: 40288274
• NCBI GeneID: 4521
• NCBI Accession #: NP_002443.3
• NCBI GenBank Nucleotide #: NM_002452.3
• UniProt Accession #: P36639; Q6LES7; Q6P0Y6; Q7Z7N6; Q8IV95; Q9UBM0; Q9UBM9; A4D205
• Molecular Weight: 17,952 Da
• NCBI Official Full Name: 7,8-dihydro-8-oxoguanine triphosphatase isoform p18
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• NCBI Official Symbol: NUDT1
• NCBI Official Synonym Symbols: MTH1
• NCBI Protein Information: 7,8-dihydro-8-oxoguanine triphosphatase; 2-hydroxy-dATP diphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-dGTPase; mutT human homolog 1; nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1; nudix motif 1
• UniProt Protein Name: 7,8-dihydro-8-oxoguanine triphosphatase
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT1
• UniProt Synonym Gene Names: MTH1; Nudix motif 1
• UniProt Entry Name: 8ODP_HUMAN

NCBI Description

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]

Uniprot Description

NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Hydrolase; EC 3.6.1.56; EC 3.6.1.55

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol

Molecular Function: GTPase activity; snoRNA binding; m7G(5')pppN diphosphatase activity; ATP diphosphatase activity; metal ion binding; 8-oxo-7,8-dihydroguanine triphosphatase activity

Biological Process: DNA protection; dGTP catabolic process; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine nucleotide catabolic process; response to oxidative stress; DNA repair