MTH1 Recombinant Protein | NUDT1 recombinant protein

MTH1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8122061

• Gene Names: NUDT1; MTH1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: MTH1; MTH1 Protein; Human; Recombinant (His Tag); Human NUDT1/MTH1 Protein (His Tag); nudix (nucleoside diphosphate linked moiety X)-type motif 1; NUDT1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val156

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human NUDT1 (NP_002443.3)(Met1-Val156) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for NUDT1 recombinant protein

Background: NUDT1 (Nudix Hydrolase 1) is a Protein Coding gene. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates to monophosphates, thereby preventing misincorporation. The NUDT1 protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Cancers can survive the oxidative conditions by upregulating nucleoside diphosphate linked moiety X-type motif 1 (NUDT1). MiR-485-5p acts as a tumor suppressor by targeting NUDT1 in gastric cancer (GC). The miR-485-5p/NUDT1 axis is involved in the processes of cell growth and cell motility and plays a key role in the tumorigenesis of GC.

NCBI and Uniprot Product Information

• NCBI GI #: 40288274
• NCBI GeneID: 4521
• NCBI Accession #: NP_002443.3
• NCBI GenBank Nucleotide #: NM_002452.3
• UniProt Accession #: P36639; Q6LES7; Q6P0Y6; Q7Z7N6; Q8IV95; Q9UBM0; Q9UBM9; A4D205
• Molecular Weight: 17,952 Da
• NCBI Official Full Name: 7,8-dihydro-8-oxoguanine triphosphatase isoform p18
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• NCBI Official Symbol: NUDT1
• NCBI Official Synonym Symbols: MTH1
• NCBI Protein Information: 7,8-dihydro-8-oxoguanine triphosphatase; 2-hydroxy-dATP diphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-dGTPase; mutT human homolog 1; nucleoside diphosphate-linked moiety X motif 1; nucleosi
• UniProt Protein Name: 7,8-dihydro-8-oxoguanine triphosphatase
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT1
• UniProt Synonym Gene Names: MTH1; Nudix motif 1
• UniProt Entry Name: 8ODP_HUMAN

NCBI Description

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]

Uniprot Description

NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Hydrolase; EC 3.6.1.56; EC 3.6.1.55

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol

Molecular Function: GTPase activity; snoRNA binding; m7G(5')pppN diphosphatase activity; ATP diphosphatase activity; metal ion binding; 8-oxo-7,8-dihydroguanine triphosphatase activity

Biological Process: DNA protection; dGTP catabolic process; dATP catabolic process; nucleobase, nucleoside and nucleotide metabolic process; purine nucleotide catabolic process; response to oxidative stress; DNA repair