NuDT1 recombinant protein

NuDT1, 1-156aa, Human, His tag, E Coli

Cat. Num. AAA203533

• Gene Names: NUDT1; MTH1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NuDT1; 1-156aa; Human; His tag; E Coli; 78-dihydro-8-oxoguanine triphosphatase; MTH1; 8-oxo-dGTPase; Nudix motif 1; 7; 8-dihydro-8-oxoguanine triphosphatase; NuDT1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl
• Concentration: 1mg/ml (determined by BCA) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV
• Sequence Length: 156

• Applicable Applications for NuDT1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NuDT1 recombinant protein

NuDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. It is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. And this protein expressed at much higher levels in proliferating cells than in resting cells. Recombinant human NuDT1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NuDT1 recombinant protein

Enzymes & Proteases

References

Satoh J., et al (2000) Eur J Neurol. 7 (6):673-7.; Oda H., et al. (1997) J. Biol. Chem. 272:17843-17850;

NCBI and Uniprot Product Information

• NCBI GI #: 40288284
• NCBI GeneID: 4521
• NCBI Accession #: NP_945191
• NCBI GenBank Nucleotide #: NM_198953.1
• UniProt Accession #: P36639; Q6LES7; Q6P0Y6; Q7Z7N6; Q8IV95; Q9UBM0; Q9UBM9; A4D205
• Molecular Weight: 20.1kDa (176aa), confirmed by MALDI-TOF
• NCBI Official Full Name: 7,8-dihydro-8-oxoguanine triphosphatase isoform p18
• NCBI Official Synonym Full Names: nudix hydrolase 1
• NCBI Official Symbol: NUDT1
• NCBI Official Synonym Symbols: MTH1
• NCBI Protein Information: 7,8-dihydro-8-oxoguanine triphosphatase
• UniProt Protein Name: 7,8-dihydro-8-oxoguanine triphosphatase
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT1
• UniProt Synonym Gene Names: MTH1; Nudix motif 1

NCBI Description

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.