TrkC / NTRK3 recombinant protein

Recombinant Human TrkC / NTRK3 Protein (His tag)

Cat. Num. AAA2546565

• Gene Names: NTRK3; TRKC; GP145-TrkC; gp145(trkC)
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TrkC / NTRK3; Recombinant Human TrkC / NTRK3 Protein (His tag); NTRK3; TrkC; gp145; TrkC / NTRK3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 612

• Application Notes: The recombinant human TrkC consists of 408 amino acids and has a calculated molecular mass of 46.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 80-90 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Cys 32
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TrkC / NTRK3 recombinant protein

Background: NT-3 growth factor receptor also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudatoputamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4. In the PNS, trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.

Description: A DNA sequence encoding the extracellular domain (Met 1-Asp 428) of human TrkC (NP_001007157.1) was expressed with a C-terminal polyhistide tag.

NCBI and Uniprot Product Information

• NCBI GI #: 55956794
• NCBI GeneID: 4916
• NCBI Accession #: NP_001007157.1
• NCBI GenBank Nucleotide #: NM_001007156.2
• UniProt Accession #: Q16288; O75682; Q12827; Q16289; B7Z4C5; E9PG56; H0YND1
• Molecular Weight: 91,833 Da
• NCBI Official Full Name: NT-3 growth factor receptor isoform c
• NCBI Official Synonym Full Names: neurotrophic receptor tyrosine kinase 3
• NCBI Official Symbol: NTRK3
• NCBI Official Synonym Symbols: TRKC; GP145-TrkC; gp145(trkC)
• NCBI Protein Information: NT-3 growth factor receptor
• UniProt Protein Name: NT-3 growth factor receptor
• UniProt Gene Name: NTRK3
• UniProt Synonym Gene Names: TRKC; Trk-C

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

TrkC: a receptor tyrosine kinase of the TRK family. Receptor for neurotrophin-3 (NT-3). Known substrates for the trk receptors are SHC, PI-3 kinase, and PLCG1. Four alternatively spliced isoforms are known.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: receptor complex

Molecular Function: neurotrophin binding; neurotrophin receptor activity; p53 binding; protein binding

Biological Process: activation of MAPK activity; activation of protein kinase B; heart development; negative regulation of protein amino acid phosphorylation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of peptidyl-serine phosphorylation; positive regulation of positive chemotaxis; positive regulation of protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway