Typical Testing Data/Standard Curve (for reference only)
Rat Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit | NTRK2 elisa kit
Rat Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit
Reactivity
Rat
Synonyms
Neurotrophic Tyrosine Kinase Receptor Type 2; Rat Neurotrophic Tyrosine Kinase Receptor Type 2 ELISA Kit; NTRK2 elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000 pg/mL
Sensitivity
1.0pg/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NTRK2 elisa kit
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This SOX1 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SOX1 in the sample, this SOX1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SOX1 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of SOX1 utilizes a multiclonal anti-SOX1 antibody and an SOX1-HRP conjugate. The assay sample and buffer are incubated together with SOX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOX1 concentration since SOX1 from samples and SOX1-HRP conjugate compete for the anti-SOX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOX1 from the sample, fewer sites are left to bind SOX1-HRP conjugate. Standards of known SOX1 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of SOX1. The SOX1 concentration in each sample is interpolated from this standard curve.
Intended Uses: This SOX1 ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of SOX1 in the sample, this SOX1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus SOX1 concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of SOX1 utilizes a multiclonal anti-SOX1 antibody and an SOX1-HRP conjugate. The assay sample and buffer are incubated together with SOX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOX1 concentration since SOX1 from samples and SOX1-HRP conjugate compete for the anti-SOX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SOX1 from the sample, fewer sites are left to bind SOX1-HRP conjugate. Standards of known SOX1 concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of SOX1. The SOX1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for NTRK2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
91,999 Da
UniProt Protein Name
Tyrosine-protein kinase receptor
Protein Family
UniProt Gene Name
NTRK2
UniProt Entry Name
Q548C2_HUMAN
Similar Products
Product Notes
The Rat NTRK2 ntrk2 (Catalog #AAA726122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726122 ELISA Kit recognizes Rat NTRK2. It is sometimes possible for the material contained within the vial of "Neurotrophic Tyrosine Kinase Receptor Type 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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