Vesicle-fusing ATPase Recombinant Protein | NSF recombinant protein

Recombinant Rat Vesicle-fusing ATPase

Cat. Num. AAA9422038

• Reactivity: Rat
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Vesicle-fusing ATPase; Recombinant Rat Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion proteinVesicular-fusion protein NSF; NSF recombinant protein

• Host: E Coli
• Reactivity: Rat
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial of the Full Length of 1-744aa, 7-209aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for NSF recombinant protein

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Ses to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 mbrane cycling.

NCBI and Uniprot Product Information

• NCBI GI #: 13489067
• NCBI GeneID: 60355
• NCBI Accession #: NP_068516.1
• NCBI GenBank Nucleotide #: NM_021748.1
• UniProt Accession #: Q9QUL6
• Molecular Weight: 26.6kD
• NCBI Official Full Name: vesicle-fusing ATPase
• NCBI Official Synonym Full Names: N-ethylmaleimide sensitive factor, vesicle fusing ATPase
• NCBI Official Symbol: Nsf
• NCBI Protein Information: vesicle-fusing ATPase
• UniProt Protein Name: Vesicle-fusing ATPase
• Protein Family: Vesicle-fusing ATPase
• UniProt Gene Name: Nsf
• UniProt Synonym Gene Names: Erg1; NEM-sensitive fusion protein

NCBI Description

binds AMPA receptor GluR2 subunit and acts to disrupt GluR2-PICK1 interactions; may play a role in AMPA receptor trafficking and stabilization [RGD, Feb 2006]

Uniprot Description

NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: Adaptor/scaffold; Autophagy; EC 3.6.4.6; Protease; Vesicle

Chromosomal Location of Human Ortholog: 10q32.1

Cellular Component: cytosol; dendritic shaft; Golgi stack; lysosomal membrane; myelin sheath; plasma membrane; postsynaptic density

Molecular Function: ATP-dependent protein binding; ATPase activity; ATPase activity, coupled; D1 dopamine receptor binding; ionotropic glutamate receptor binding; PDZ domain binding; protein binding; protein C-terminus binding; protein complex binding; protein kinase binding; Rab GTPase binding; SNARE binding; syntaxin binding; syntaxin-1 binding

Biological Process: intracellular protein transport; neurotransmitter secretion; positive regulation of protein catabolic process; positive regulation of receptor recycling; potassium ion transport; protein transport; regulation of exocytosis; vesicle fusion; vesicle-mediated transport