Rabbit FBL Polyclonal Antibody | anti-FBL antibody

FBL (Fibrillarin, FIB, FLRN, RNU3IP1) (PE)

Cat. Num. AAA6416980

• Gene Names: FBL; FIB; FLRN; RNU3IP1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: FBL; Polyclonal Antibody; FBL (Fibrillarin; FIB; FLRN; RNU3IP1) (PE); anti-FBL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes FBL. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-FBL antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human FBL.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FBL antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using FBL antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using FBL antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using FBL antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-FBL antibody

Antibodies; Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 31395
• NCBI GeneID: 2091
• UniProt Accession #: P22087; O75259; Q6IAT5; Q9UPI6; B5BUE8
• Molecular Weight: 33,784 Da
• NCBI Official Full Name: fibrillarin
• NCBI Official Synonym Full Names: fibrillarin
• NCBI Official Symbol: FBL
• NCBI Official Synonym Symbols: FIB; FLRN; RNU3IP1
• NCBI Protein Information: rRNA 2'-O-methyltransferase fibrillarin; 34-kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Protein Family: rRNA 2'-O-methyltransferase fibrillarin
• UniProt Gene Name: FBL
• UniProt Synonym Gene Names: FIB1; FLRN
• UniProt Entry Name: FBRL_HUMAN

NCBI Description

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Uniprot Description

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: RNA-binding; RNA processing; Methyltransferase; Nucleolus; EC 2.1.1.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing