DNA-directed RNA polymerase D subunit 2a (NRPD2a) Recombinant Protein | NRPD2a recombinant protein

Recombinant Arabidopsis thaliana DNA-directed RNA polymerase D subunit 2a (NRPD2a) , partial

• Gene Names: NRPD2A; DEFECTIVE IN MERISTEM SILENCING 2; DMS2; DRD2; NRPD2; NRPE2; nuclear RNA polymerase D2A; OCP1; OVEREXPRESSOR OF CATIONIC PEROXIDASE 1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase D subunit 2a (NRPD2a); Recombinant Arabidopsis thaliana DNA-directed RNA polymerase D subunit 2a (NRPD2a); partial; NRPD2a recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,172

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 334185578
• NCBI GeneID: 821960
• NCBI Accession #: NP_001189957.1
• NCBI GenBank Nucleotide #: NM_001203028.2
• UniProt Accession #: Q9LK40; Q67ZK6
• Molecular Weight: 132,654 Da
• NCBI Official Full Name: nuclear RNA polymerase D2A
• NCBI Official Symbol: NRPD2A
• NCBI Official Synonym Symbols: DEFECTIVE IN MERISTEM SILENCING 2; DMS2; DRD2; NRPD2; NRPE2; nuclear RNA polymerase D2A; OCP1; OVEREXPRESSOR OF CATIONIC PEROXIDASE 1
• NCBI Protein Information: nuclear RNA polymerase D2A
• UniProt Protein Name: DNA-directed RNA polymerases IV and V subunit 2
• UniProt Gene Name: NRPD2
• UniProt Synonym Gene Names: DMS2; DRD2; NRPD2a; NRPE2; RPD2a; AtNRPD2a; Nuclear RNA polymerase D 2a; POL IV 2a

NCBI Description

This gene encodes the second largest, catalytic subunit of the nuclear DNA-dependent RNA polymerase IV (aka RNA polymerase D). The NRPD2 protein is found at nuclear foci that overlap or are adjacent to chromocentromeres but are not fully coincident with chromocentromeres. The loss of NRPD2 leads to the loss of cytosine methylation at pericentromeric 5S genes and AtSN1 retroelements but has no discernible effect on centromere repeat methylation. This suggests that Pol IV primarily affects facultative heterochromatin rather than constitutive heterochromatin.

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Also required for full erasure of methylation when the RNA trigger is withdrawn. Required for intercellular RNA interference (RNAi) leading to systemic post-transcriptional gene silencing. Involved in the maintenance of post-transcriptional RNA silencing. During interphase, mediates siRNA-independent heterochromatin association and methylation into chromocenters and condensation and cytosine methylation at pericentromeric major repeats. Required for complete maintenance of the 35S promoter homology-dependent TGS in transgenic plants and for the initial establishment of DNA methylation.