Rat Collagen Type II Alpha 1 ELISA Kit | COL2a1 elisa kit

Rat Collagen Type II Alpha 1 ELISA Kit

Cat. Num. AAA730412

• Gene Names: COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
• Reactivity: Rat
• Synonyms: Collagen Type II Alpha 1; Rat Collagen Type II Alpha 1 ELISA Kit; COL2a1 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/ml
• Intended Uses: This CoL-2A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CoL-2A1. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL2a1 elisa kit

Principle of the Assay: CoL-2A1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CoL-2A1. Standards or samples are then added to the microtiter plate wells and CoL-2A1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CoL-2A1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CoL-2A1 are added to each well to "sandwich" the CoL-2A1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CoL-2A1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-2A1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for COL2a1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 30041
• NCBI GeneID: 1280
• UniProt Accession #: P02458; Q12985; Q14009; Q14044; Q14045; Q14046; Q14047; Q14056; Q14058; Q16672; Q1JQ82; A6NGA0
• Molecular Weight: 29,781 Da
• NCBI Official Full Name: COL2A1, partial
• NCBI Official Synonym Full Names: collagen, type II, alpha 1
• NCBI Official Symbol: COL2A1
• NCBI Official Synonym Symbols: AOM; ANFH; SEDC; STL1; COL11A3
• NCBI Protein Information: collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; arthroophthalmopathy, progressive (Stickler syndrome)
• UniProt Protein Name: Collagen alpha-1(II) chain
• UniProt Gene Name: COL2A1
• UniProt Entry Name: CO2A1_HUMAN

NCBI Description

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; collagen type II; extracellular region; basement membrane

Molecular Function: identical protein binding; platelet-derived growth factor binding; metal ion binding; extracellular matrix structural constituent conferring tensile strength

Biological Process: heart morphogenesis; axon guidance; proteoglycan metabolic process; extracellular matrix organization and biogenesis; inner ear morphogenesis; central nervous system development; collagen fibril organization; palate development; notochord development; collagen catabolic process; limb bud formation; extracellular matrix disassembly; sensory perception of sound; visual perception; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; skeletal development; endochondral ossification; cartilage condensation

Disease: Achondrogenesis, Type Ii; Kniest Dysplasia; Spondyloperipheral Dysplasia; Epiphyseal Dysplasia, Multiple, With Myopia And Conductive Deafness; Legg-calve-perthes Disease; Osteoarthritis With Mild Chondrodysplasia; Stickler Syndrome, Type I; Czech Dysplasia; Platyspondylic Lethal Skeletal Dysplasia, Torrance Type; Otospondylomegaepiphyseal Dysplasia; Spondyloepimetaphyseal Dysplasia, Strudwick Type; Avascular Necrosis Of Femoral Head, Primary; Spondyloepiphyseal Dysplasia Congenita; Stickler Syndrome, Type I, Nonsyndromic Ocular