Mouse Myosin-1 (MYH1) ELISA Kit | MYH1 elisa kit

Mouse Myosin-1 (MYH1) ELISA Kit

Cat. Num. AAA7215467

• Gene Names: MYH1; MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
• Reactivity: Mouse
• Synonyms: Myosin-1 (MYH1); Mouse Myosin-1 (MYH1) ELISA Kit; MYH1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This MYH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MYH1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH1 elisa kit

Principle of the assay: MYH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYH1 antibody and an MYH1-HRP conjugate. The assay sample and buffer are incubated together with MYH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYH1 concentration since MYH1 from samples and MYH1-HRP conjugate compete for the anti-MYH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYH1 from the sample, fewer sites are left to bind MYH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYH1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MYH1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 115527082
• NCBI GeneID: 4619
• NCBI Accession #: NP_005954.3
• NCBI GenBank Nucleotide #: NM_005963.3
• Molecular Weight: 223,145 Da
• NCBI Official Full Name: myosin-1
• NCBI Official Synonym Full Names: myosin, heavy chain 1, skeletal muscle, adult
• NCBI Official Symbol: MYH1
• NCBI Official Synonym Symbols: MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
• NCBI Protein Information: myosin-1; myHC-IIx/d; myosin heavy chain 1; myosin heavy chain 2x; myosin heavy chain IIx/d; epididymis luminal protein 71; myosin heavy chain, skeletal muscle, adult 1; myosin, heavy polypeptide 1, skeletal muscle, adult
• UniProt Protein Name: Myosin-1
• UniProt Gene Name: MYH1
• UniProt Synonym Gene Names: MyHC-2x; MyHC-IIx/d
• UniProt Entry Name: MYH1_HUMAN

NCBI Description

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]

Uniprot Description

MYH1: Muscle contraction.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: muscle myosin complex; A band

Molecular Function: calmodulin binding; motor activity; actin binding; ATP binding

Biological Process: muscle contraction; metabolic process