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SDS-Page

Neuropeptide Y (NPY) Recombinant Protein | NPY recombinant protein

Recombinant Neuropeptide Y (NPY)

Gene Names
Npy; NPY02; RATNPY; RATNPY02
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Neuropeptide Y (NPY); Recombinant Neuropeptide Y (NPY); NPY recombinant protein
Ordering
For Research Use Only!
Host
E. coli
Purity/Purification
> 90%
Form/Format
PBS, pH7.4, containing 0.01% SKL.
Concentration
Original Concentration: 200ug/mL (varies by lot)
Sequence
PSKPDNPGED APAEDMARYY SALRHYINLI TRQRYGKRSS PETLISDLLM RESTENAPRT RLEDPSMW
Applicable Applications for NPY recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Pro31~Trp98
Tags
N-terminal His and GST Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
6.4
Usage
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 37.8kDa
Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
pro-neuropeptide Y preproprotein
NCBI Official Synonym Full Names
neuropeptide Y
NCBI Official Symbol
Npy
NCBI Official Synonym Symbols
NPY02; RATNPY; RATNPY02
NCBI Protein Information
pro-neuropeptide Y
UniProt Protein Name
Pro-neuropeptide Y
Protein Family
UniProt Gene Name
Npy
UniProt Synonym Gene Names
NPY; CPON
UniProt Entry Name
NPY_RAT

NCBI Description

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. Studies in the rat model of depression (Flinders Sensitive Line) show that this gene is downregulated in the hippocampus and the prefrontal cortex compared to the control ((Flinders Resistant Line). Alternatively spliced transcript variants of this gene have been described, but the function of all the variants is not known. [provided by RefSeq, Jul 2012]

Uniprot Description

NPY: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Belongs to the NPY family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Cellular Component: Golgi apparatus; extracellular space; cytoplasm

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding; neuropeptide Y receptor binding; neuropeptide Y receptor activity

Biological Process: synaptic transmission; sensory perception of taste; neuropeptide signaling pathway; positive regulation of appetite; regulation of blood pressure; adult feeding behavior; feeding behavior; cerebral cortex development; negative regulation of blood pressure; neurite development; central nervous system neuron development

Research Articles on NPY

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Product Notes

The NPY npy (Catalog #AAA2009380) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Neuropeptide Y (NPY) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the NPY npy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PSKPDNPGED APAEDMARYY SALRHYINLI TRQRYGKRSS PETLISDLLM RESTENAPRT RLEDPSMW. It is sometimes possible for the material contained within the vial of "Neuropeptide Y (NPY), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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