Human Fibroblast Activation Protein Alpha ELISA Kit | FAPalpha elisa kit

Human Fibroblast Activation Protein Alpha ELISA Kit

Cat. Num. AAA766049

• Gene Names: FAP; FAPA; SIMP; DPPIV
• Reactivity: Human
• Synonyms: Fibroblast Activation Protein Alpha; Human Fibroblast Activation Protein Alpha ELISA Kit; FAPalpha/FAP/Seprase/170 kDa melanoma membrane-bound gelatinase/DKFZp686G13158/DPPIV/EC 3.4.21.-/FAPA/Fibroblast activation protein alpha/fibroblast activation protein; alpha/Integral membrane serine protease/seprase; FAPalpha elisa kit

• Reactivity: Human
• Sequence Length: 760

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FAPalpha elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human FAPalpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FAPalpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FAPalpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FAPalpha can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119631758
• NCBI GeneID: 2191
• Molecular Weight: 26,954 Da
• NCBI Official Full Name: fibroblast activation protein, alpha, isoform CRA_a
• NCBI Official Synonym Full Names: fibroblast activation protein alpha
• NCBI Official Symbol: FAP
• NCBI Official Synonym Symbols: FAPA; SIMP; DPPIV
• NCBI Protein Information: prolyl endopeptidase FAP
• UniProt Protein Name: Prolyl endopeptidase FAP
• UniProt Gene Name: FAP
• UniProt Synonym Gene Names: APCE
• UniProt Entry Name: SEPR_HUMAN

NCBI Description

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Uniprot Description

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.26; Membrane protein, integral; EC 3.4.14.5

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: apical part of cell; cell surface; cytoplasm; extracellular space; focal adhesion; integral to membrane; lamellipodium; plasma membrane

Molecular Function: dipeptidyl-peptidase activity; endopeptidase activity; integrin binding; metalloendopeptidase activity; peptidase activity; protease binding; protein binding; protein dimerization activity; protein homodimerization activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: angiogenesis; cell adhesion; endothelial cell migration; proteolysis; proteolysis involved in cellular protein catabolic process; regulation of fibrinolysis