Human Atrial natriuretic peptide receptor 1 ELISA Kit | NPR1 elisa kit

Human Atrial natriuretic peptide receptor 1 ELISA Kit

Cat. Num. AAA760109

• Gene Names: NPR1; ANPa; NPRA; ANPRA; GUC2A; GUCY2A
• Reactivity: Human
• Synonyms: Atrial natriuretic peptide receptor 1; Human Atrial natriuretic peptide receptor 1 ELISA Kit; NPR1 (Atrial natriuretic peptide receptor 1)/ANPa/ANPRA/GC-A/GUCY2A/NPRA/Pndr/ANP-A/ANPR-A/Atrial natriuretic peptide receptor type A/atrionatriuretic peptide receptor A/Guanylate cyclase A/GUC2A/natriuretic peptide A type receptor/natriuretic peptide receptor A/natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptidereceptor A)/NPR-A; NPR1 elisa kit

• Reactivity: Human
• Sequence Length: 1061

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NPR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NPR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NPR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NPR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NPR1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 167830411
• NCBI GeneID: 4881
• NCBI Accession #: NP_000897.3
• NCBI GenBank Nucleotide #: NM_000906.3
• Molecular Weight: 118,919 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 1
• NCBI Official Synonym Full Names: natriuretic peptide receptor 1
• NCBI Official Symbol: NPR1
• NCBI Official Synonym Symbols: ANPa; NPRA; ANPRA; GUC2A; GUCY2A
• NCBI Protein Information: atrial natriuretic peptide receptor 1
• UniProt Protein Name: Atrial natriuretic peptide receptor 1
• Protein Family: Atrial natriuretic peptide receptor
• UniProt Gene Name: NPR1
• UniProt Synonym Gene Names: ANPRA; ANP-A; ANPR-A; NPR-A; GC-A
• UniProt Entry Name: ANPRA_HUMAN

NCBI Description

Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]

Uniprot Description

ANPA: natriuretic peptide receptor A, which mediates natriuretic, diuretic, and vasorelaxing actions of the natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Has guanylate cyclase activity on binding of ANF. Hypertensive SHR Rat has polymorphism at the locus. Human non-coding polymorphisms can alter expression up to two-fold, and have been associated with hypertension. A coding SNP is associated with hypertension and myocardial infarction.

Protein type: Protein kinase, RGC; Kinase, protein; Membrane protein, integral; EC 4.6.1.2; Lyase; Receptor, misc.; Nucleotide Metabolism - purine; Protein kinase, dual-specificity (receptor); Guanylyl cyclase; RGC group; RGC family

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: guanylate cyclase complex, soluble; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: adenylate cyclase activity; ATP binding; GTP binding; guanylate cyclase activity; hormone binding; natriuretic peptide receptor activity; peptide hormone binding; peptide receptor activity, G-protein coupled; protein kinase activity; protein kinase binding

Biological Process: body fluid secretion; cell surface receptor linked signal transduction; cGMP biosynthetic process; dopamine metabolic process; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of smooth muscle cell proliferation; positive regulation of cGMP biosynthetic process; protein amino acid phosphorylation; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of vascular permeability; regulation of vasodilation