Immunohistochemistry (IHC)
(Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit anti-Human HAUS8 Polyclonal Antibody | anti-HAUS8 antibody
Anti-HAUS8 Antibody
IHC-P: 5 ug/ml
WB: 0.5 ug/ml
Long Term: Aliquot and store at -20 degree C
Avoid freeze thaw cycles.
Immunohistochemistry (IHC)
(Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-HAUS8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Western Blot of Rabbit anti-HAUS8 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 ul per lane. Primary antibody: HAUS8 antibody at 1.24 ug/mL overnight at 4 degree C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40, 000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HAUS8. Other band(s): HAUS8 splice variants and isoforms.)
Western Blot (WB)
(Western Blot of Rabbit anti-HAUS8 antibody. Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 ul per lane. Primary antibody: HAUS8 antibody at 1.24 ug/mL overnight at 4 degree C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40, 000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 48 kDa, 48 kDa for HAUS8. Other band(s): HAUS8 splice variants and isoforms.)
Western Blot (WB)
(Western blot of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with DYKDDDDK X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with DYKDDDDK X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.)
Western Blot (WB)
(Western blot of Rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with DYKDDDDK X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with DYKDDDDK X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.)
NCBI and Uniprot Product Information
NCBI Description
HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM, Jun 2010]
Uniprot Description
Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Research Articles on HAUS8
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Product Notes
The HAUS8 haus8 (Catalog #AAA2400993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HAUS8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HAUS8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:30000-1:45000 IHC-P: 5 ug/ml WB: 0.5 ug/ml. Researchers should empirically determine the suitability of the HAUS8 haus8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAUS8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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