Mouse C-type natriuretic peptide ELISA Kit | Nppc elisa kit

Mouse C-type natriuretic peptide ELISA Kit

Cat. Num. AAA288759

• Gene Names: Nppc; CNP; lbab
• Reactivity: Mouse
• Synonyms: C-type natriuretic peptide; Mouse C-type natriuretic peptide ELISA Kit; Nppc; Cnp; Nppc elisa kit

• Reactivity: Mouse
• Sequence Length: 126

• Assay Type: Competitive
• Detection Range: 15.6-1000 pg/mL
• Sensitivity: 8.01 pg/mL
• Intra-Assay CV: <=5.4%
• Inter-Assay CV: <=8.9%
• Recovery: 97%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Nppc elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6754880
• NCBI GeneID: 18159
• NCBI Accession #: NP_035063.1
• NCBI GenBank Nucleotide #: NM_010933.5
• UniProt Accession #: Q61839
• Molecular Weight: 13,320 Da
• NCBI Official Full Name: C-type natriuretic peptide preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide type C
• NCBI Official Symbol: Nppc
• NCBI Official Synonym Symbols: CNP; lbab
• NCBI Protein Information: C-type natriuretic peptide
• UniProt Protein Name: C-type natriuretic peptide
• Protein Family: C-type natriuretic peptide
• UniProt Gene Name: Nppc
• UniProt Synonym Gene Names: Cnp
• UniProt Entry Name: ANFC_MOUSE

NCBI Description

This gene encodes a member of the natriuretic peptide family. Natriuretic peptides are involved in the control of blood pressure, extracellular fluid volume and electrolyte homeostasis. The encoded protein also plays a role in sensory neuron bifurcation, and is a critical regulator of endochondral bone growth. The encoded protein is a ligand for the natriuretic peptide receptor B, and is synthesized as a preprohormone which is cleaved to produce a mature peptide. Mutations in this gene are associated with dwarfism resulting from impaired endochondral ossification. [provided by RefSeq, Apr 2011]

Uniprot Description

NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Cellular Component: extracellular region; extracellular space; secretory granule

Molecular Function: hormone activity; neuropeptide hormone activity; peptide hormone receptor binding; protein homodimerization activity; receptor binding

Biological Process: cGMP biosynthetic process; negative regulation of cell proliferation; negative regulation of DNA metabolic process; negative regulation of meiotic cell cycle; organ development; ossification; positive regulation of cGMP biosynthetic process; positive regulation of osteoblast differentiation; positive regulation of vasodilation; post-embryonic development; receptor guanylyl cyclase signaling pathway; regulation of blood vessel size; regulation of cAMP metabolic process; regulation of cGMP metabolic process; regulation of multicellular organism growth