Human C-type natriuretic peptide ELISA Kit | NPPC elisa kit

Human C-type natriuretic peptide ELISA Kit

Cat. Num. AAA762717

• Gene Names: NPPC; CNP; CNP2
• Reactivity: Human
• Synonyms: C-type natriuretic peptide; Human C-type natriuretic peptide ELISA Kit; NPPC/CNP/CNP2/C-type natriuretic peptide/natriuretic peptide C/natriuretic peptide precursor C; NPPC elisa kit

• Reactivity: Human
• Sequence Length: 126

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NPPC elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human NPPC. During the reaction,Human NPPC in the sample or standard competes with a fixed amount of Human NPPC on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Human NPPC. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human NPPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13249346
• NCBI GeneID: 4880
• NCBI Accession #: NP_077720.1
• NCBI GenBank Nucleotide #: NM_024409.3
• Molecular Weight: 13,246 Da
• NCBI Official Full Name: C-type natriuretic peptide preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide C
• NCBI Official Symbol: NPPC
• NCBI Official Synonym Symbols: CNP; CNP2
• NCBI Protein Information: C-type natriuretic peptide
• UniProt Protein Name: C-type natriuretic peptide
• Protein Family: C-type natriuretic peptide
• UniProt Gene Name: NPPC
• UniProt Synonym Gene Names: CNP2
• UniProt Entry Name: ANFC_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cardiac natriuretic peptides CNP-53, CNP-29 and CNP-22, which belong to the natriuretic family of peptides. The encoded peptides exhibit vasorelaxation activity in laboratory animals and elevated levels of CNP-22 have been observed in the plasma of chronic heart failure patients. [provided by RefSeq, Oct 2015]

Uniprot Description

NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted, signal peptide; Hormone; Secreted

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: extracellular region; extracellular space; secretory granule

Molecular Function: hormone activity; peptide hormone receptor binding; protein homodimerization activity; receptor binding

Biological Process: cGMP biosynthetic process; negative regulation of cell proliferation; negative regulation of DNA metabolic process; negative regulation of meiotic cell cycle; ossification; positive regulation of cGMP biosynthetic process; positive regulation of osteoblast differentiation; positive regulation of vasodilation; post-embryonic development; receptor guanylyl cyclase signaling pathway; regulation of cAMP metabolic process; regulation of cGMP metabolic process; regulation of multicellular organism growth; regulation of smooth muscle cell proliferation; response to drug; response to ethanol; response to hypoxia