Typical Testing Data/Standard Curve
Canine C-Type Natriuretic Peptide ELISA Kit | CNP elisa kit
Canine C-Type Natriuretic Peptide ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||CNP ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CNP antibody and an CNP-HRP conjugate. The assay sample and buffer are incubated together with CNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNP concentration since CNP from samples and CNP-HRP conjugate compete for the anti-CNP antibody binding site. Since the number of sites is limited, as more sites are occupied by CNP from the sample, fewer sites are left to bind CNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is proteolytically processed to form a secreted hormone of the natriuretic peptide family. The encoded hormone regulates the growth and differentiation of cartilaginous growth plate chondrocytes and may also be vasoactive and natriuretic. [provided by RefSeq, Sep 2011]
Uniprot Description
NPPC: Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.
Protein type: Secreted; Hormone; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: extracellular space; secretory granule
Molecular Function: protein homodimerization activity; peptide hormone receptor binding; hormone activity; receptor binding
Biological Process: response to drug; cGMP biosynthetic process; ossification; regulation of smooth muscle cell proliferation; regulation of cAMP metabolic process; regulation of multicellular organism growth; negative regulation of meiotic cell cycle; negative regulation of DNA metabolic process; positive regulation of vasodilation; post-embryonic development; negative regulation of cell proliferation; positive regulation of osteoblast differentiation; response to ethanol; positive regulation of cGMP biosynthetic process; receptor guanylyl cyclase signaling pathway; response to hypoxia; regulation of cGMP metabolic process; regulation of blood vessel size
Research Articles on CNP
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Product Notes
The Canine CNP nppc (Catalog #AAA735187) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA735187 ELISA Kit recognizes Canine CNP. It is sometimes possible for the material contained within the vial of "C-Type Natriuretic Peptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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